Abstract:
Glioblastoma represents the most aggressive tumor of the central nervous system. Due to invasion of glioblastoma
stem cells into the healthy tissue, chemoresistance, and recurrence of the tumor, it is difficult to successfully treat glioblastoma
patients, which is demonstrated by the low life expectancy of patients after standard therapy treatment. Recently, we found that
diisothiocyanate-derived mercapturic acids, which are isothiocyanate derivatives from plants of the Cruciferae family, provoked a
decrease in glioblastoma cell viability. These findings were extended by combining diisothiocyanate-derived mercapturic acids with
dinaciclib (a small-molecule inhibitor of cyclin-dependent kinases with anti-proliferative capacity) or temozolomide (TMZ, standard
chemotherapeutic agent) to test whether the components have a cytotoxic effect on glioblastoma cells when the dosage is low. Here,
we demonstrate that the combination of diisothiocyanate-derived mercapturic acids with dinaciclib or TMZ had an additive or even
synergistic effect in the restriction of cell growth dependent on the combination of the components and the glioblastoma cell source.
This strategy could be applied to inhibit glioblastoma cell growth as a therapeutic interference of glioblastoma.