Development of Spectral Imaging Cytometry
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Methods in Molecular Biology
Abstract
Abstract Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells—from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry.
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Keywords
Quantum mechanics, Computational chemistry, Geology, Molecular biology, Spectroscopy, Physics, Biology, Remote sensing, Chemistry, Optics, Materials science, Biological system, Spectral properties, Spectral analysis, Cytometry, Hyperspectral imaging, Spectral imaging, Fluorescence, Flow cytometry, Autofluorescence
Citation
Ivan A. Vorobjev, Aigul Kussanova, & Natasha S. Barteneva (2023). Development of Spectral Imaging Cytometry. . https://doi.org/10.1007/978-1-0716-3020-4_1