Development of Spectral Imaging Cytometry

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dc.contributor.authorNatasha S. Barteneva
dc.contributor.authorAigul Kussanova
dc.contributor.authorIvan A. Vorobjev
dc.date.accessioned2025
dc.date.issued2023
dc.description.abstractAbstract Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells—from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry.
dc.identifier.citationIvan A. Vorobjev, Aigul Kussanova, & Natasha S. Barteneva (2023). Development of Spectral Imaging Cytometry. . https://doi.org/10.1007/978-1-0716-3020-4_1
dc.identifier.doi10.1007/978-1-0716-3020-4_1
dc.identifier.urihttps://doi.org/10.1007/978-1-0716-3020-4_1
dc.identifier.urihttps://nur.nu.edu.kz/handle/123456789/17430
dc.languageen
dc.publisherMethods in Molecular Biology
dc.rightsAll rights reserved
dc.sourceMethods in Molecular Biology
dc.subjectQuantum mechanics
dc.subjectComputational chemistry
dc.subjectGeology
dc.subjectMolecular biology
dc.subjectSpectroscopy
dc.subjectPhysics
dc.subjectBiology
dc.subjectRemote sensing
dc.subjectChemistry
dc.subjectOptics
dc.subjectMaterials science
dc.subjectBiological system
dc.subjectSpectral properties
dc.subjectSpectral analysis
dc.subjectCytometry
dc.subjectHyperspectral imaging
dc.subjectSpectral imaging
dc.subjectFluorescence
dc.subjectFlow cytometry
dc.subjectAutofluorescence
dc.titleDevelopment of Spectral Imaging Cytometry
dc.typeArticle

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