Browsing by Author "Kushugulova, A."

Now showing 1 - 7 of 7
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    Analysis of protector mechanism of probiotics using DNA comet assay
    (Nazarbayev University, 2014) Zhulamanova, A.; Shakhabayeva, G.; Saduakhasova, S.; Kozhakhmetov, S.; Kushugulova, A.
    Ecology affects human organism by causing breaks in DNA and, therefore, induce mutations and malignant cell formation. Comet assay is a quantitative method that allows to measure DNA damage in eukaryotic cells (Liao, McNutt, & Zhu, 2009). This method is widely used in areas, such as human biomonitoring, genotoxicology, and ecology. Comet assay is also called a single-cell gel electrophoresis. Thus, the products of electrophoresis are analyzed under the fluorescent light, which makes it similar to comets. DNA strand breaks are detected by comparing the intensity of the comet tail relative to the head (Collins, 2004). Probiotics containing Lactobacillus rhamnosus were used, as Lactobacilli probiotics were found to be safe and effective against urogenital diseases, food hypersensitivity, and dental caries (Lebeer, Vanderleyden, & De Keersmaeckler, 2008). The aim of the research was to study DNA protector mechanism for three probiotic products, containing L. rhamnosus.
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    Antiradical and Cytoprotective Properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats
    (Frontiers, 2021) Sergazy, S.; Gulyayev, A.; Amangeldiyeva, A.; Nurgozhina, A.; Nurgaziyev, M.; Shulgau, Z.; Chulenbayeva, L.; Khassenbekova, Z.; Kushugulova, A.; Aljofan, M.
    The aim of this study is determine the in vitro and in vivo antiradical properties and the cytoprotective activity of Allium nutans L. honey extract. The antiradical properties of the extracts were investigated in rabbit alveolar macrophages and human foreskin fibroblast (hFFs) cells in the presence of doxorubicin, a cytotoxic substance using DPPH and ABTS assays. The cytoprotective activities were determined using 18 Wistar rats divided into three different groups, a negative control, and two other groups with experimentally induced hepatotoxicity by a single intraperitoneal injection of 50% carbon tetrachloride (CCl4) oil solution. A positive control group, received drinking water only and an experimental group that was treated with Allium nutans L. honey extracts for 7 days. In vitro treatment with Allium nutans L. honey extracts resulted in 78% reduction in radical activity in DPPH and 91.6% inhibition using the ABTS. Also, honey extracts were able to preserve 100% of cell viability in the presence of the cytotoxic, doxorubicin. Furthermore, the treatment with honey extracts resulted in a significant reduction in damage to the structure of liver tissue, as well significant reduction in the levels of ALT and AST in the experimental group compared to the control group
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    Clinical characterizations of children with bacterial meningitis (BM) in the Republic of Kazakhstan
    (2016-12-01) Seidullayeva, A.; Zhaxylykova, G.; Kenzhebayeva, S.; Turdalina, B.; Volkova, G.; Nurtazina, G.; Kostrovskaya, T.; Greenberg, D.; Kushugulova, A.; Bayesheva, D.; A., Seidullayeva
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    Effect of Celergen, a marine derivative, on in vitro hepatocarcinogenesis
    (Drug Discoveries & Therapeutics. DOI: 10.5582/ddt.2013.v7.5.196, 2013) Catanzaro, R.; Zerbinati, N.; Solimene, U.; Celep, G.; Marotta, F.; Kushugulova, A.; Milazzo, M.; Tomella, C.; Bertuccelli, G.; Zhumadilov, Z.
    The aim of this study was to test for a potential anticarcinogenic effect of Celergen, a marine derivative devoid of traceable amounts of inorganic arsenic, on cell proliferation, cell cycle progression and apoptosis in the HepG2 human liver cancer cell line. Celergen significantly inhibited the proliferation of cancer cells in a dose-dependent manner while limiting the cell cycle progression at the G1 phase and significantly inducing apoptosis. Further examination showed that Celergen enhanced expression of the p21CIPl1WAF1, GADD153 genes and downregulated the c-myc gene. These results suggest that Celergen exerts promising chemopreventive properties to be further investigated.
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    Incidence of meningococcal disease in children in Astana city
    (International Journal of Infectious Diseases, 2016-12-01) Seidullayeva, A.; Zhaxylykova, G.; Bayesheva, D.; Turdalina, B.; Balgayeva, M.; Kushugulova, A.; Greenberg, D.; Volkova, G.; A., Seidullayeva
    The main aim of present study is to evaluate the clinical and epidemiological features of meningococcal disease in children under the age of 14 in Astana for 2015.
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    PIN17 Pharmacoeconomics of Cervarix Vaccines Against Human Papilloma Virus in the Republic of Kazakhstan
    (2013-11-01) Nurgozhin, T.; Gulyaev, A.; Yermekbayeva, B.; Kushugulova, A.; Akhmetova, A.; Supiev, A.; T., Nurgozhin
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    The antimicrobial activity of honey Kazakhstan regions
    (National Laboratory Astana, Nazarbayev University, 2017-09-15) Khassenbekova, Zh.; Gulayev, A.; Akhmetova, A.; Sergazy, Sh.; Baidildinova, G.; Kushugulova, A.; Kozhakhmetov, S.; Urasova, M.; Nurgozhin, T.