Antiradical and Cytoprotective Properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats
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Date
2021
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Journal ISSN
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Publisher
Frontiers
Abstract
The aim of this study is determine the in vitro and in vivo antiradical properties and the
cytoprotective activity of Allium nutans L. honey extract. The antiradical properties of the
extracts were investigated in rabbit alveolar macrophages and human foreskin fibroblast
(hFFs) cells in the presence of doxorubicin, a cytotoxic substance using DPPH and ABTS
assays. The cytoprotective activities were determined using 18 Wistar rats divided into
three different groups, a negative control, and two other groups with experimentally
induced hepatotoxicity by a single intraperitoneal injection of 50% carbon tetrachloride
(CCl4) oil solution. A positive control group, received drinking water only and an
experimental group that was treated with Allium nutans L. honey extracts for 7 days. In
vitro treatment with Allium nutans L. honey extracts resulted in 78% reduction in radical
activity in DPPH and 91.6% inhibition using the ABTS. Also, honey extracts were able to
preserve 100% of cell viability in the presence of the cytotoxic, doxorubicin. Furthermore,
the treatment with honey extracts resulted in a significant reduction in damage to the
structure of liver tissue, as well significant reduction in the levels of ALT and AST in the
experimental group compared to the control group
Description
Keywords
cytotoxicity, doxorubicin, polyphenol, flavonoid, CCl4-induced liver damage, anti-radical activities
Citation
Sergazy, S., Gulyayev, A., Amangeldiyeva, A., Nurgozhina, A., Nurgaziyev, M., Shulgau, Z., Chulenbayeva, L., Khassenbekova, Z., Kushugulova, A., & Aljofan, M. (2021). Antiradical and Cytoprotective Properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats. Frontiers in Pharmacology, 12. https://doi.org/10.3389/fphar.2021.687763