Analysis of protector mechanism of probiotics using DNA comet assay
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Date
2014
Authors
Zhulamanova, A.
Shakhabayeva, G.
Saduakhasova, S.
Kozhakhmetov, S.
Kushugulova, A.
Journal Title
Journal ISSN
Volume Title
Publisher
Nazarbayev University
Abstract
Ecology affects human organism by causing breaks in DNA and, therefore, induce
mutations and malignant cell formation. Comet assay is a quantitative method that allows to measure
DNA damage in eukaryotic cells (Liao, McNutt, & Zhu, 2009). This method is widely used in areas,
such as human biomonitoring, genotoxicology, and ecology. Comet assay is also called a single-cell gel
electrophoresis. Thus, the products of electrophoresis are analyzed under the fluorescent light, which
makes it similar to comets. DNA strand breaks are detected by comparing the intensity of the comet
tail relative to the head (Collins, 2004). Probiotics containing Lactobacillus rhamnosus were used, as
Lactobacilli probiotics were found to be safe and effective against urogenital diseases, food hypersensitivity,
and dental caries (Lebeer, Vanderleyden, & De Keersmaeckler, 2008). The aim of the research was to
study DNA protector mechanism for three probiotic products, containing L. rhamnosus.
Description
Keywords
ecology, human organism, DNA, comet assay, eukaryotic cells, biomonitoring, genotoxicology, gel electrophoresis, electrophoresis, fluorescent light, probiotics