Browsing by Author "Gulyayev, A."

Now showing 1 - 9 of 9
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    Antioxidant properties of grape polyphenol concentrate from Kazakhstan region
    (2016-05) Yermekbayeva, B.; Shulgau, Z.; Sergazy, Sh.; Kabdullina, A.; Khassenbekova, Zh.; Tritek, V.; Abuova, G.; Zhaugasheva, S.; Gulyayev, A.; Nurgozhin, T.
    It is believed that the antioxidant effects are widespread among plant polyphenols. Antioxidant effect is associated with main biological activities of polyphenols. Objective. To describe the antioxidant effect of grape polyphenol concentrate from Kazakhstan selection in vivo on models of pathologies with overproduction of free radicals.
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    Antiradical and Cytoprotective Properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats
    (Frontiers, 2021) Sergazy, S.; Gulyayev, A.; Amangeldiyeva, A.; Nurgozhina, A.; Nurgaziyev, M.; Shulgau, Z.; Chulenbayeva, L.; Khassenbekova, Z.; Kushugulova, A.; Aljofan, M.
    The aim of this study is determine the in vitro and in vivo antiradical properties and the cytoprotective activity of Allium nutans L. honey extract. The antiradical properties of the extracts were investigated in rabbit alveolar macrophages and human foreskin fibroblast (hFFs) cells in the presence of doxorubicin, a cytotoxic substance using DPPH and ABTS assays. The cytoprotective activities were determined using 18 Wistar rats divided into three different groups, a negative control, and two other groups with experimentally induced hepatotoxicity by a single intraperitoneal injection of 50% carbon tetrachloride (CCl4) oil solution. A positive control group, received drinking water only and an experimental group that was treated with Allium nutans L. honey extracts for 7 days. In vitro treatment with Allium nutans L. honey extracts resulted in 78% reduction in radical activity in DPPH and 91.6% inhibition using the ABTS. Also, honey extracts were able to preserve 100% of cell viability in the presence of the cytotoxic, doxorubicin. Furthermore, the treatment with honey extracts resulted in a significant reduction in damage to the structure of liver tissue, as well significant reduction in the levels of ALT and AST in the experimental group compared to the control group
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    Cardioprotective properties of polyphenol concentrate in Rat model of Doxorubicin-induced Cardiomyopathy
    (2015-08-01) Shulgau, Z.; Gulyayev, A.; Yermekbayeva, B.; Adilgozhina, G.; Tritek, V.; Nurgozhin, T.; Z., Shulgau
    Present study aimed to investigate possible cardioprotective properties of polyphenol concentrates obtained from Cabernet Sauvignon type of Kazakhstani grapes using rat model doxorubicin-induced cardiomyopathy.
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    Cytisin Amidophosphate – Promising Role for The Liver Treatment
    (2015-08-01) Gulyayev, A.; Shulgau, Z.; Yermekbayeva, B.; Sergazy, S.; Nurgozhin, T.; A., Gulyayev
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    Erythrocyte ghosts` influence on the phenotypic plasticity of macrophage
    (2016-05) Khassenbekova, Zh.; Berikkhanova, K.; Gulyayev, A.; Sergazy, Sh.; Yermekbayeva, В.; Kushugulova, A.R.; Omarova, D.; Akhmetova, A.; Nurgozhin, T.; Zhumadilov, Zh.
    Correction of phenotypic plasticity of macrophages for reprogramming inflammatory response is of great interest to modern medicine. To date, there are lots of factors, which can alter macrophages' phenotype to M1 or M2. The introduction of drug containing autologous erythrocyte ghosts is one of such factors. We have developed a method for the targeted delivery of autologous erythrocyte ghosts loaded with antibiotic (pharmacocytes) directly into the wound tissues. Introduction of pharmacocytes to the purulent wound ensures high therapeutic concentrations of the antibiotic, which leads to rapid arresting of the phase of the inflammatory process and thus results in early healing. In order to explain this phenomenon, we investigated the effect of the pharmacocytes on the phenotypic polarization of macrophages.
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    Investigating the Suitability of Mare’s Milk-Derived Exosomes as Potential Drug Carriers.
    (MDPI, 2024) Sergazy, S.; Zhetkenev, S; Shulgau, Z.; Chulenbayeva, L.; Kamyshanskiy, Y.; Nurgaziyev, M.; Nurgozhina, A.; Mukhanbetzhanova, Z.; Berikkhanova, K.; Gulyayev, A.; Aljofan M.
    Exosomes are cell-derived, membrane-surrounded particles that deliver bioactive molecules to various cells. Due to their small size, low immunogenicity, extended blood circulation, and involvement in cellular communication, they hold potential as effective drug carriers. Exosomes are present in various biological fluids, including mare’s milk, a traditional drink in Central Asia. This study aims to compare exosome isolation methodologies and determine the stability of mare’s milk-derived exosomes as potential therapeutic carriers. Three extraction methods—immunoprecipitation, size exclusion chromatography, and total exosome isolation—were compared in terms of exosome characteristics, purity, and content. The isolated exosomes were then loaded with quercetin, and their ability to increase its bioavailability was tested in vitro and in vivo. Total exosome isolation was identified as the most efficient method for producing high-quality exosomes. These exosomes were loaded with quercetin and compared to free quercetin and exosomes alone. Exosomes loaded with 80 µM quercetin significantly restored β-galactosidase activity and cellular viability in doxorubicin-treated cells, exhibiting similar potency to 160 µM free quercetin. In aged model animals, treatment with quercetin-loaded exosomes resulted in significantly less acute and subacute damage to the myocardium, kidneys, and liver compared to untreated control animals. This study provides a proof-of-concept that mare’s milk-derived exosomes can be effectively absorbed by cells and animal tissues, supporting their potential use as drug carriers.
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    Pharmacokinetics of Ceftriaxone Included In Cellular Transport System
    (2015-08-01) Nurgozhin, T.; Gulyayev, A.; Lokhvytsky, S.; Yermekbayeva, B.; Sergazy, S.; Shulgau, Z.; Berikkhanova, K.; T., Nurgozhin
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    Possible Changes of Pharmacokinetics of IL-2 Deposited in Red Blood Cells (RBC)
    (2017-08-01) Tritek, V.; Zhaugasheva, S.K.; Gulyayev, A.; Sergazy, Sh.; Khassenbekova, Zh.; Yermekbayeva, B.; Nurgozhin, T.; V., Tritek
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    The Protective Properties of the Extract of Polyphenols from Grapes of Cabernet Sauvignon Varieties In The Model of Acute Radiation Sickness in Rats
    (2017-08-01) Sergazy, Sh.; Shulgau, Z.; Gulyayev, A.; Tritek, V.; Adilgozhina, G.; Khassenbekova, Zh.; Nugozhin, T.; Sh., Sergazy
    To investigate possible protective effect of the polyphenol concentrate from Cabernet Sauvignon collection of grapes in rat model of acute radiation sickness (ARS).