STUDYING CYTOTOXIC EFFECTS OF CYCLOHEXIMIDE ON COLON CANCER CELLS

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dc.contributor.authorKalen, Aibibi
dc.date.accessioned2021-08-16T07:06:49Z
dc.date.available2021-08-16T07:06:49Z
dc.date.issued2021-06-28
dc.description.abstractCycloheximide (CHX) is reported to cause apoptosis in a variety of cells. Previous studies show that CHX can sensitize the cells to TNF-α-induced apoptosis in T lymphocyte cells, leukocytes, liver cancer cells and fibroblasts. However, a mechanism behind the cytotoxicity of CHX is not completely described. In present work, cytotoxic effects of CHX on colorectal adenocarcinoma cells Caco-2 were examined. CHX triggered cell death and the viability of Caco-2 cells decreased in a dose-dependent fashion. One hypothesis is that CHX causes production of reactive oxygen species that are tightly involved in several apoptotic pathways. ROS formation after treating Caco-2 cells with 0, 5, 15 and 30 μg/ml CHX was evaluated by a confocal microscope. The cells were stained with a fluorogenic MitoSOX dye that specifically targets mitochondria. MitoSOX is readily oxidized by ROS and the product of a such reaction shows fluorescence, which allows to stain mitochondria only when there is a ROS production. Caco-2 cells were positive for MitoSOX indicating that CHX treatment causes ROS formation. Furthermore, fluorescence intensity measurements displayed that ROS were produced in a dose-dependent manner. Future studies should focus on studying how ROS production is involved in CHX-mediated cell deathen_US
dc.identifier.citationKalen, A. (2021). Studying cytotoxic effects of cycloheximide on colon cancer cells (Unpublished master`s thesis). Nazarbayev University, Nur-sultan, Kazakhstanen_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/5686
dc.language.isoenen_US
dc.publisherNazarbayev University School of Sciences and Humanitiesen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectType of access: Gated Accessen_US
dc.subjectCycloheximideen_US
dc.subjectcancer cellsen_US
dc.titleSTUDYING CYTOTOXIC EFFECTS OF CYCLOHEXIMIDE ON COLON CANCER CELLSen_US
dc.typeMaster's thesisen_US
workflow.import.sourcescience

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