STUDYING CYTOTOXIC EFFECTS OF CYCLOHEXIMIDE ON COLON CANCER CELLS
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Date
2021-06-28
Authors
Kalen, Aibibi
Journal Title
Journal ISSN
Volume Title
Publisher
Nazarbayev University School of Sciences and Humanities
Abstract
Cycloheximide (CHX) is reported to cause apoptosis in a variety of cells. Previous studies
show that CHX can sensitize the cells to TNF-α-induced apoptosis in T lymphocyte cells,
leukocytes, liver cancer cells and fibroblasts. However, a mechanism behind the cytotoxicity
of CHX is not completely described. In present work, cytotoxic effects of CHX on colorectal
adenocarcinoma cells Caco-2 were examined. CHX triggered cell death and the viability of
Caco-2 cells decreased in a dose-dependent fashion. One hypothesis is that CHX causes
production of reactive oxygen species that are tightly involved in several apoptotic pathways.
ROS formation after treating Caco-2 cells with 0, 5, 15 and 30 μg/ml CHX was evaluated by
a confocal microscope. The cells were stained with a fluorogenic MitoSOX dye that
specifically targets mitochondria. MitoSOX is readily oxidized by ROS and the product of a
such reaction shows fluorescence, which allows to stain mitochondria only when there is a
ROS production. Caco-2 cells were positive for MitoSOX indicating that CHX treatment
causes ROS formation. Furthermore, fluorescence intensity measurements displayed that
ROS were produced in a dose-dependent manner. Future studies should focus on studying
how ROS production is involved in CHX-mediated cell death
Description
Keywords
Type of access: Gated Access, Cycloheximide, cancer cells
Citation
Kalen, A. (2021). Studying cytotoxic effects of cycloheximide on colon cancer cells (Unpublished master`s thesis). Nazarbayev University, Nur-sultan, Kazakhstan