USING THE DYNAMIC PLATFORM FOR THE DETECTION OF CANCER BIOMARKER CD44

Abstract

Early detection of CD44 cancer biomarker is essential because overexpression of the transmembrane protein CD44 in the human body is a signal for the growth and progression of tumors. Clinical studies confirmed that CD44 is considered useful in prognozing and diagnosing several types of cancer, such as gastric, colon, breast and oral cancer. Therefore, accurate and easy-to-use CD44 biomarker detection methods can significantly assist in cancer diagnosis and control disease conditions. This conducted study aims to develop an accurate dynamic biosensing platform for detecting CD44 biomarker to mimic blood flow, thus allowing to move one step closer to the clinical application. In vitro studies were done after optimizing the dynamic setup, and sucrose solution measurement was performed in static and dynamic conditions to see the difference and resolve issues before protein measurement. SMF-28 fibers were utilized to manufacture optical fiber ball resonators in the lab, and these fabricated optical fiber ball resonator biosensors were designed to detect CD44 protein. CD44 protein detection and specificity tests were done with several functionalized biosensors in dynamic conditions. Developed optical fiber biosensor demonstrated its competence in the detection of CD44 biomarker.