PLANT EXTRACT OF LIMONIUM GMELINII ATTENUATES OXIDATIVE RESPONSES IN NEURONS, ASTROCYTES, AND CEREBRAL ENDOTHELIAL CELLS IN VITRO AND IMPROVES MOTOR FUNCTIONS OF RATS AFTER MIDDLE CEREBRAL ARTERY OCCLUSION

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dc.contributor.authorNurkenov, Tulendy
dc.contributor.authorTsoy, Andrey
dc.contributor.authorOlzhayev, Farkhad
dc.contributor.authorAbzhanova, Elvira
dc.contributor.authorTurgambayeva, Anel
dc.contributor.authorZhussupova, Aizhan
dc.contributor.authorAvula, Bharathi
dc.contributor.authorRoss, Samir
dc.contributor.authorAituarova, Aigerim
dc.contributor.authorKassymova, Dariya
dc.contributor.authorZhusupova, Galiya
dc.contributor.authorShalakhmetova, Tamara
dc.contributor.authorTokay, Tursonjan
dc.contributor.authorLee, James C.
dc.contributor.authorAskarova, Sholpan
dc.date.accessioned2021-12-20T03:00:32Z
dc.date.available2021-12-20T03:00:32Z
dc.date.issued2021-11-15
dc.description.abstractThere are numerous publications demonstrating that plant polyphenols can reduce oxidative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of L. gmelinii since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H2O2 , and TNF-α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P-selectin expression, and activity of ERK1/2 were evaluated by quantitative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF-α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF-α induced oxidative stress and triggered the accumulation of P-selectin on the surface of the cells. In turn, pre-treatment of the cells with the extract of L. gmelinii suppressed oxidative stress in all cell types and pro-inflammatory responses in astrocytes and CECs. In vivo, the treatment with L. gmelinii extract improved motor activity in rats with MCAO. Keywords: antioxidants; neurons; astrocytes; CECs; L. gmelinii; plant polyphenolsen_US
dc.identifier.citationNurkenov, T., Tsoy, A., Olzhayev, F., Abzhanova, E., Turgambayeva, A., Zhussupova, A., Avula, B., Ross, S., Aituarova, A., Kassymova, D., Zhusupova, G., Shalakhmetova, T., Tokay, T., Lee, J. C., & Askarova, S. (2021). Plant Extract of Limonium gmelinii Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion. In Antioxidants (Vol. 10, Issue 11, p. 1814). MDPI AG. https://doi.org/10.3390/antiox10111814en_US
dc.identifier.issn2076-3921
dc.identifier.urihttps://doi.org/10.3390/antiox10111814
dc.identifier.urihttps://www.mdpi.com/2076-3921/10/11/1814
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/5931
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofseriesAntioxidants;2021, 10, 1814. https://doi.org/10.3390/antiox10111814
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectantioxidantsen_US
dc.subjectneuronsen_US
dc.subjectastrocytesen_US
dc.subjectType of access: Open Accessen_US
dc.subjectCECsen_US
dc.subjectL. gmeliniien_US
dc.subjectplant polyphenolsen_US
dc.titlePLANT EXTRACT OF LIMONIUM GMELINII ATTENUATES OXIDATIVE RESPONSES IN NEURONS, ASTROCYTES, AND CEREBRAL ENDOTHELIAL CELLS IN VITRO AND IMPROVES MOTOR FUNCTIONS OF RATS AFTER MIDDLE CEREBRAL ARTERY OCCLUSIONen_US
dc.typeArticleen_US
workflow.import.sourcescience

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