PLANT EXTRACT OF LIMONIUM GMELINII ATTENUATES OXIDATIVE RESPONSES IN NEURONS, ASTROCYTES, AND CEREBRAL ENDOTHELIAL CELLS IN VITRO AND IMPROVES MOTOR FUNCTIONS OF RATS AFTER MIDDLE CEREBRAL ARTERY OCCLUSION
Loading...
Date
2021-11-15
Authors
Nurkenov, Tulendy
Tsoy, Andrey
Olzhayev, Farkhad
Abzhanova, Elvira
Turgambayeva, Anel
Zhussupova, Aizhan
Avula, Bharathi
Ross, Samir
Aituarova, Aigerim
Kassymova, Dariya
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
There are numerous publications demonstrating that plant polyphenols can reduce oxidative stress and inflammatory processes in the brain. In the present study we have investigated
the neuroprotective effect of plant extract isolated from the roots of L. gmelinii since it contains a
rich source of polyphenols and other biologically active compounds. We have applied an oxidative
and inflammatory model induced by NMDA, H2O2
, and TNF-α in human primary neurons and
astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation,
NADPH oxidase activation, P-selectin expression, and activity of ERK1/2 were evaluated by quantitative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor
functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA
induced overproduction of ROS, in astrocytes TNF-α initiated ROS generation, NADPH oxidase
activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF-α induced oxidative stress
and triggered the accumulation of P-selectin on the surface of the cells. In turn, pre-treatment of the
cells with the extract of L. gmelinii suppressed oxidative stress in all cell types and pro-inflammatory
responses in astrocytes and CECs. In vivo, the treatment with L. gmelinii extract improved motor
activity in rats with MCAO.
Keywords: antioxidants; neurons; astrocytes; CECs; L. gmelinii; plant polyphenols
Description
Keywords
antioxidants, neurons, astrocytes, Type of access: Open Access, CECs, L. gmelinii, plant polyphenols
Citation
Nurkenov, T., Tsoy, A., Olzhayev, F., Abzhanova, E., Turgambayeva, A., Zhussupova, A., Avula, B., Ross, S., Aituarova, A., Kassymova, D., Zhusupova, G., Shalakhmetova, T., Tokay, T., Lee, J. C., & Askarova, S. (2021). Plant Extract of Limonium gmelinii Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion. In Antioxidants (Vol. 10, Issue 11, p. 1814). MDPI AG. https://doi.org/10.3390/antiox10111814