DEVELOPMENT OF AN OXYGEN SENSOR INTEGRATED MICROFLUIDICS AND ITS ONLINE MONITORING SYSTEM
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Date
2024
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Nazarbayev University School of Engineering and Digital Sciences
Abstract
This paper designs and analyzes a microfluidic oxygen sensor toward the development of organ-on-chip (OoC) technology. It was designed to establish a system that allows real-time observation of oxygen uptake
rate by mammalian cells, highly important during the investigation of changes in behavior and viability of cells under different conditions. This project developed and fabricated a microfluidic chip with cell precision monitoring using frontier microfluidic technology and fiber optic sensing. A 3D CAD software-designed chip, integrated with a PICO-O2 oxygen sensor developed by PyroScience GmbH, and an experimental setup have been calibrated and tested with respect to controlled experimental conditions carried out. Calibration and testing of the developed experimental setup were carried out for controlled experimental conditions, and therefore it is very important for the reliability and accuracy of data. Data was collected at a continuous level, measuring the O2 level and cellular responses using PyroScience Workbench for comprehensive visualized plotting. The results from the experiments indicated that the chip could support a viable cell culture; in addition, the cell culture measured oxygen uptake accurately. This really does illustrate the potential to provide tremendous insight into cellular dynamics, which are essential for the success of most biomedical research and therapeutic development, from such integrated systems. They further illustrate that, to help translate this out of the laboratory and into real applications, really high-precision engineering and calibration were going to be needed. This advance does not set any limit for organ-on-chip technologies but rather allows opening new venues in cellular biology research, where in due course, importance has to be given to real-time data acquisition. Further works can be done to integrate more numbers of sensing elements and scale the system to make it work for broader applications.
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type of access: open access, microfluidic oxygen sensor
Citation
Tsoy, Y. (2024). Development of an oxygen sensor integrated microfluidics and its online monitoring system. Nazarbayev University School of Engineering and Digital Sciences