Imaging flow cytometry for phytoplankton analysis

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dc.contributor.authorDashkova, Veronika
dc.contributor.authorMalashenkov, Dmitry
dc.contributor.authorPoulton, Nicole
dc.contributor.authorVorobjev, Ivan
dc.contributor.authorBarteneva, Natasha S.
dc.creatorVeronika, Dashkova
dc.date.accessioned2017-12-22T08:50:29Z
dc.date.available2017-12-22T08:50:29Z
dc.date.issued2017-01-01
dc.description.abstractAbstract This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities’ composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.en_US
dc.identifierDOI:10.1016/j.ymeth.2016.05.007
dc.identifier.citationVeronika Dashkova, Dmitry Malashenkov, Nicole Poulton, Ivan Vorobjev, Natasha S. Barteneva, Imaging flow cytometry for phytoplankton analysis, In Methods, Volume 112, 2017, Pages 188-200en_US
dc.identifier.citationViraga Haridas, Shahin Ranjbar, Ivan A. Vorobjev, Anne E. Goldfeld, Natasha S. Barteneva, Imaging flow cytometry analysis of intracellular pathogens, In Methods, Volume 112, 2017, Pages 91-104
dc.identifier.issn10462023
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S104620231630130X
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3052
dc.language.isoenen_US
dc.publisherMethodsen_US
dc.relation.ispartofMethods
dc.rights.license© 2016 Elsevier Inc. All rights reserved.
dc.subjectMicroalgaeen_US
dc.subjectPhytoplanktonen_US
dc.subjectImaging flow cytometryen_US
dc.subjectViabilityen_US
dc.subjectMetabolic activityen_US
dc.titleImaging flow cytometry for phytoplankton analysisen_US
dc.typeArticleen_US
dcterms.publisherMethods
elsevier.aggregationtypeJournal
elsevier.coverdate2017-01-01
elsevier.coverdisplaydate1 January 2017
elsevier.endingpage200
elsevier.identifier.doi10.1016/j.ymeth.2016.05.007
elsevier.identifier.eid1-s2.0-S104620231630130X
elsevier.identifier.piiS1046-2023(16)30130-X
elsevier.identifier.scopusid84973885584
elsevier.issue.nameFlow Cytometry
elsevier.openaccess0
elsevier.openaccessarticlefalse
elsevier.openarchivearticlefalse
elsevier.startingpage188
elsevier.teaserThis review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining...
elsevier.volume112
workflow.import.sourcescience

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