Imaging flow cytometry for phytoplankton analysis

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Date

2017-01-01

Authors

Dashkova, Veronika
Malashenkov, Dmitry
Poulton, Nicole
Vorobjev, Ivan
Barteneva, Natasha S.

Journal Title

Journal ISSN

Volume Title

Publisher

Methods

Abstract

Abstract This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities’ composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.

Description

Keywords

Microalgae, Phytoplankton, Imaging flow cytometry, Viability, Metabolic activity

Citation

Veronika Dashkova, Dmitry Malashenkov, Nicole Poulton, Ivan Vorobjev, Natasha S. Barteneva, Imaging flow cytometry for phytoplankton analysis, In Methods, Volume 112, 2017, Pages 188-200
Viraga Haridas, Shahin Ranjbar, Ivan A. Vorobjev, Anne E. Goldfeld, Natasha S. Barteneva, Imaging flow cytometry analysis of intracellular pathogens, In Methods, Volume 112, 2017, Pages 91-104

URI

https://www.sciencedirect.com/science/article/pii/S104620231630130X
 http://nur.nu.edu.kz/handle/123456789/3052

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