GUT MODULATION OF DYSBIOSIS INDUCED BY DEXTRAN SULFATE SODIUM
Loading...
Date
2021-06-08
Authors
Kozhakhmetov, Samat S.
Babenko, Dmitriy
Kozhakhmetova, Saniya
Tuyakova, Altynay
Nurgaziyev, Madiyar
Nurgozhina, Ayaulym
Muhanbetganov, Nurislam
Chulenbayeva, Laura
Sergazy, Shynggys
Gulyayev, Alexander
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Ltd
Abstract
Inflammatory bowel disease is one of the serious burdens of clinical medicine and healthcare. This study investigated the potential of a biological product based on mare's milk and metabolites of symbiotic microflora for modulation of intestinal microflora affected by dextran sulfate sodium (DSS)-induced dysbiosis. Symbiotic microflora was isolated from the stool of healthy volunteers. Lysates for the production of short-chain fatty acids of screened microorganisms were mixed with mare's milk. The activity of the biological product was evaluated on the DSS model of induced colitis. Histological changes in the intestinal epithelium were determined. The structure of the microbiome was evaluated based on the analysis of 16S rRNA microbial sequences. Histological examination of rat intestinal tissues after application of the biological product showed reduced infiltration of granulocytes, macrophages, and lymphocytes. The results of sequencing demonstrated a decrease in the biological diversity of microbiota affected by colitis. The full recovery was observed after 21 days of the application of the biological product. The product induced the structural changes of the microbiome damaged by DSS. Likewise, the number of the pathogenic intestinal microflora was decreased Representatives of SCFA producing bacteria increased concentrations of genus Lactobacillus.
Description
Keywords
Type of access: Open Access, Biological product, DSS-Induced colitis, Gut microbiome, Microbial diversity, Rats
Citation
Kozhakhmetov, S., Babenko, D., Kozhakhmetova, S., Tuyakova, A., Nurgaziyev, M., Nurgozhina, A., Muhanbetganov, N., Chulenbayeva, L., Sergazy, S., Gulyayev, A., Tanabayev, B., Saliev, T., & Kushugulova, A. (2021). Gut modulation of dysbiosis induced by dextran sulfate sodium. Food Bioscience, 42, 101167. https://doi.org/10.1016/j.fbio.2021.101167