DIFFERENTIAL GENE EXPRESSION ANALYSIS BETWEEN ELITE ATHLETES AND SEDENTARY INDIVIDUALS
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Date
2024-04-03
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Nazarbayev University School of Medicine
Abstract
This study examined gene expression differences between elite athletes and sedentary individuals, exploring the genetic basis of physical activity. Utilizing "transcriptomic analysis and physical activity" among other keywords, 35 datasets were analyzed with the GEO2R tool, identifying 7,314 exercise-related genes. The most frequent nine genes (ATF3, NR4A2, NR4A3, NFIL3, SOCS3, SIK1, SLC2A3, MYC, MAFF) were further studied using the STRING database and qPCR analysis, with HBB as the qPCR normalization reference. Ethical approval was obtained, and the study involved 10 participants (5 triathletes, 5 sedentary) with no chronic health issues. Blood samples were collected, and RNA was extracted for qPCR analysis. The results showed that the mean Ct values did not differ significantly between the groups; however, variances were observed in the expression of the NR4A3 and NR4A2 genes. Additionally, fold change (log2fc) calculations revealed a four-fold decrease in NR4A2 and a two-fold decrease in the SLC2A3 gene in athletes compared to sedentary individuals.
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Type of access: Gated
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Babatola, T. A.