Аннотация:
Genotype variation in viruses can afect the response of antiviral treatment. Several studies have
established approaches to determine genotype-specifc variations; however, analyses to determine
the efect of these variations on drug–protein interactions remain unraveled. We present an in-silico
approach to explore genotype-specifc variations and their efect on drug–protein interaction. We
have used HCV NS3 helicase and fuoroquinolones as a model for drug–protein interaction and have
investigated the efect of amino acid variations in HCV NS3 of genotype 1a, 1b, 2b and 3a on NS3fuoroquinolone interaction. We retrieved 687, 667, 101 and 248 nucleotide sequences of HCV NS3
genotypes 1a, 1b, 2b, and 3a, respectively, and translated these into amino acid sequences and used
for genotype variation analysis, and also to construct 3D protein models for 2b and 3a genotypes. For
1a and 1b, crystal structures were used. Drug–protein interactions were determined using molecular
docking analyses. Our results revealed that individual genotype-specifc HCV NS3 showed substantial
sequence heterogeneity that resulted in variations in docking interactions. We believe that our
approach can be extrapolated to include other viruses to study the clinical signifcance of genotypespecifc variations in drug–protein interactions.