Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery
Loading...
Date
2019
Authors
Sultankulov, B.
Berillo, D.
Kauanova, S.
Mikhalovsky, S.
Mikhalovska, L.
Saparov, A.
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Macroporous scaffolds composed of chitosan (CHI), hydroxyapatite (HA), heparin (Hep), and polyvinyl alcohol (PVA) were prepared with a glutaraldehyde (GA) cross-linker by cryogelation. Addition of PVA to the reaction mixture slowed down the formation of a polyelectrolyte complex (PEC) between CHI and Hep, which allowed more thorough mixing, and resulted in the development of the homogeneous matrix structure. Freezing of the CHI-HA-GA and PVA-Hep-GA mixture led to the formation of a non-stoichiometric PEC between oppositely charged groups of CHI and Hep, which caused further efficient immobilization of bone morphogenic protein 2 (BMP-2) possible due to electrostatic interactions. It was shown that the obtained cryogel matrix released BMP-2 and supported the differentiation of rat bone marrow mesenchymal stem cells (rat BMSCs) into the osteogenic lineage. Rat BMSCs attached to cryogel loaded with BMP-2 and expressed osteocalcin in vitro. Obtained composite cryogel with PEC may have high potential for bone regeneration and tissue engineering applications.
Description
Keywords
biomaterials, cryogel, drug delivery, growth factors, tissue engineering, bone regeneration, Research Subject Categories::MEDICINE
Citation
Sultankulov, B., Berillo, D., Kauanova, S., Mikhalovsky, S., Mikhalovska, L., & Saparov, A. (2019). Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery. Pharmaceutics, 11(12), 650. https://doi.org/10.3390/pharmaceutics11120650