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Browsing 02.National Laboratory Astana by Author "c4d9756f-bcde-4be0-957a-37a985471d8b"

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DESIGNING ALLELE-SPECIFIC COMPETITIVE-EXTENSION PCR-BASED ASSAYS FOR HIGH-THROUGHPUT GENOTYPING AND GENE CHARACTERIZATION

Kalendar, Ruslan; Shustov, Alexandr V.; Akhmetollayev, Ilyas; Kairov, Ulykbek (Frontiers in Molecular Biosciences, 2022)

Polymerase chain reaction (PCR) is a simple and rapid method that can detect nucleotide polymorphisms and sequence variation in basic research applications, agriculture, and medicine. Variants of PCR, collectively known ...
MODIFIED “ALLELE-SPECIFIC QPCR” METHOD FOR SNP GENOTYPING BASED ON FRET

Kalendar, Ruslan; Baidyussen, Akmaral; Serikbay, Dauren; Zotova, Lyudmila; Khassanova, Gulmira; Kuzbakova, Marzhan; Jatayev, Satyvaldy; Hu, Yin-Gang; Schramm, Carly; Anderson, Peter A.; Jenkins, Colin L. D.; Soole, Kathleen L.; Shavrukov, Yuri (Frontiers in Plant Science, 2022)

The proposed method is a modified and improved version of the existing “Allele-specific q-PCR” (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). ...
SCREENING A COLLECTION OF LOCAL AND FOREIGN VARIETIES OF SOLANUM LYCOPERSICUM L. IN KAZAKHSTAN FOR GENETIC MARKERS OF RESISTANCE AGAINST THREE TOMATO VIRUSES

Pozharskiy, Alexandr; Kostyukova, Valeriya; Taskuzhina, Aisha; Nizamdinova, Gulnaz; Kisselyova, Nina; Kalendar, Ruslan; Karimov, Nurlybek; Gritsenko, Dilyara (Heliyon, 2022)

The tomato is one of the most important vegetable crops. The successful development of tomato cultures in Kazakhstan depends on the implementation of intensive agricultural methods, including breeding and selecting for ...
THE STRUCTURAL DIVERSITY OF CACTA TRANSPOSONS IN GENOMES OF CHENOPODIUM (AMARANTHACEAE, CARYOPHYLLALES) SPECIES: SPECIFC TRAITS AND COMPARISON WITH THE SIMILAR ELEMENTS OF ANGIOSPERMS

Belyayev, Alexander; Josefová, Jiřina; Jandová, Michaela; Kalendar, Ruslan; Mahelka, Václav; Mandák, Bohumil; Krak, Karol (Mobile DNA, 2022)

Background: CACTA transposable elements (TEs) comprise one of the most abundant superfamilies of Class 2 (cut and-paste) transposons. Over recent decades, CACTA elements were widely identifed in species from the plant, ...