Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors

Abstract

In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclonal anti-L1R antibody targeting the L1R protein, and the polyclonal rabbit serum antibodies targeting the whole vaccinia virus (VV) pathogen. Experimental measurements were carried out to detect VV in concentrations from 10

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Rakhimbekova Aida, Kudaibergenov Baizak, Seitkamal Kuanysh, Bellone Aurora, Dauletova Ayazhan, Sypabekova Marzhan, Olivero Massimo, Perrone Guido, Radaelli Antonia, Zanotto Carlo, De Giuli Morghen Carlo, Vangelista Luca, Tosi Daniele. (2023). Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors. Scientific Reports. https://doi.org/https://doi.org/10.1038/s41598-023-44926-6

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