Browsing by Author "Aljofan Mohamad"

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    Antioxidant and cytoprotective properties of quercetin-loaded mare’s milk exosomes
    (Wiley, 2023) Sergazy Shynggys; Zhetkenev Sanzhar; Chulenbayeva Laura; Aljofan Mohamad; Gulyayev Alexandr
    Mare's milk is the national drink of the indigenous population of Kazakhstan. Similar to other milk types, mare's milk is a rich source of EVs, which can be loaded with flavonoids such as quercetin that may have some valuable therapeutic properties. In this study, we examine cytoprotective properties of quercetin-loaded exosomes (ExoQuer) on doxorubicin induced cellular toxicity as well as antioxidant and radical scavenging activity
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    Antiproliferative and Cytotoxic Activity of Geraniaceae Plant Extracts Against Five Tumor Cell Lines
    (Taylor & Francis, 2021) Sergazy Shynggys; Vetrova Anastassiya; Orhan Ilkay Erdogan; Senol Deniz Fatma Sezer; Kahraman Ahmet; Zhang Jian_Ye; Aljofan Mohamad
    Aim: To determine the antiproliferative and cytotoxic activities of Geranium and Erodium species against human cancer and noncancer cell lines, respectively. Methods: Twenty-one species of Geranium and Erodium were extracted and screened against cancerous and noncancerous human cell lines. Results: In a dose-response manner, G. glaberrimum, G. asphodeloides, E. brandianum and E. leucanthum were able, with variable potency, to inhibit cellular proliferation. Except for E. brandianum, all extracts induced cellular autophagy in tumor cells with similar levels to that of rapamycin; but, only E. brandianum induced cellular apoptosis, likely through Bcl2 and BAX protein expressions. Discussion: This is the first study to report the potential antiproliferative effects of ethanol extracts of several Geraniaceae species. Lay abstract Several plant species of the genera Geranium and Erodium were extracted and screened against five tumor cell lines for their potential antitumor activities. Out of the tested species, four showed potential antitumor action against all the tumor cells used. Interestingly, they appeared to achieve their antitumor activities by inducing important cellular defense mechanisms called autophagy and apoptosis. The current work is the first to test the antiproliferative potential of these species against different tumor cells.
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    Blueberry and cranberry extracts mitigate CCL4-induced liver damage, suppressing liver fibrosis, inflammation and oxidative stress.
    (Elsevier, 2023) Sergazy Shynggys; Shulgau Zarina; Kamyshanskiy Yevgeniy; Zhumadilov Zhaxybay; Krivyh Elena; Gulyayev Alexander; Aljofan Mohamad
    The current study aims to evaluate potential hepatoprotective effect of lingonberry, cranberry and blueberry polyphenols on carbon tetrachloride (CCL-4)-induced acute and subacute liver injury in rats. A total of 55 male Wistar rats, divided into six experimental and control groups. With the exception of the negative control group, all groups received an intraperitoneal injection of CCl-4, twice a week for 14 days. An extract of lingonberry, cranberry, blueberry polyphenols and the positive control, silymarin were administered daily via intragastric route, for 14 consecutive days. The untreated control group showed characteristic of classical oxidative stress-mediated liver damage with vacuolization of the hepatocyte cytoplasm, infiltration by immune cells and proliferation of collagen fibers, decrease in body weight and increase in liver weight; increased levels of AST and ALT in serum, an increased lipid peroxidation in the liver. However, the use of cranberry and blueberry polyphenols significantly suppressed liver damage, exerting an effect comparable to the hepatoprotective effect of the positive control. The extracts prevented and reduced inflammatory liver damage by reducing IL-6, TNF-α and IFN-γ levels. In conclusion, blueberry and cranberry extracts have a protective effect against acute and subacute CCl4-induced hepatotoxicity in rats.
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    Establishment and validation of mare’s milk-derived exosomes extraction method
    (Wiley, 2023) Nurgozhina Ayaulym; Sergazy Shynggys; Chulenbayeva Laura; Zhetkenev Sanzhar; Aljofan Mohamad
    extracellular vesicles, drug delivery, drug discovery, biopharmaceuticals
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    Proteomic and transcriptomic characterization of mare’s milk derived exosomes
    (Wiley, 2023) Nurgaziyev Madiyar; Nurgozhina Ayaulym; Sergazy Shynggys; Gulyayev Alexandr; Chulenbayeva Laura; Zhetkenev Sanzhar; Aljofan Mohamad
    Extracellular vesicles, drug delivery, drug discovery, biopharmaceuticals