RESULTS OF THE AUTOLOGOUS MESENCHYMAL STEM CELL TRANSPLANTATION IN PATIENTS WITH TYPE 1DIABETES MELLITUS

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Date

2020

Authors

Ulyanova, O.
Baigenzhin, A.
Askarov, M.
Kozina, L.
Karibekov, T.
Taubaldieva, Zh.
Serebrennikova, D.
Boltanova, A.

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International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana

Abstract

Introduction: Type 1diabetes mellitus (T1DM) remains one of the main public healthcare problems worldwide with a tendency to steady growth. T1DM leads to kidney failure, blindness, heart attacks and strokes, which account for high rates of morbidity and mortality among patients with T1DM. A replacement of beta cells is the goal of therapy for T1DM. Recent clinical studies have shown a promising stem cell role in the treatment of T1DM. We evaluated the therapeutic effect of autologous mesenchymal stem cell transplantation (AMSCT) on carbohydrate metabolism markers in T1DM patients. Methods: We examined 7 patients (5 male, 2female, aged 20-42) with T1DM, who underwent AMSCT (cells were obtained from the patients’ iliac crest and cultivated for 3-4 weeks) by intravenous infusion. The quantity of autologous mesenchymal stem cells infused was from 95 to 97 × 106. We analyzed the daily insulin dosages, glycated hemoglobin (HbA1c), glutamic acid decarboxylase (GAD) antibody and Langerhans antibody levels in patients before, 1, 2and 3 months after the AMSCT procedure. Results: In patients with T1DM, AMSCT led to decrease in daily insulin dosage levels from 58,81± 13,71Units to 47,5± 12,7 Units (p=0,04) with trend to increase leptin levels and decrease HbA1c levels, from 7,73 + 3.5 ng/ml to 16,9 ± 8,31ng/ml (p= 0,046) and 9,59±1,73% to 8,65±0,93 % (p = 0,092) after 1month, respectively. GAD antibody and Langerhans antibody levels didn’t change significantly after AMSCT: from 10,79 + 4,52IU/ml to 12,33 ± 3,81IU/ml (p> 0,05) and 14,12+4,26 IU/mL to 18,17+9,03 IU/mL (р=0,485) after 3 months, respectively. Conclusions: The AMSCT led to decrease of the daily insulin dosage levels with increase of the leptin levels after 1month without increasing of the GAD and Langerhans antibody levels within 3 months in patients with T1DM.

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Keywords

Autologous bone-marrow derived stem cells, Type1diabetes mellitus, Glycated hemoglobin, Research Subject Categories::MEDICINE

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