ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins

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dc.contributor.authorAl-Ismaeel, Qais
dc.contributor.authorNeal, Christopher P.
dc.contributor.authorAl-Mahmoodi, Hanaa
dc.contributor.authorAlmutairi, Zamzam
dc.contributor.authorAl-Shamarti, Ibtihal
dc.contributor.authorStraatman, Kees
dc.contributor.authorJaunbocus, Nabil
dc.contributor.authorIrvine, Andrew
dc.contributor.authorIssa, Eyad
dc.contributor.authorMoreman, Catherine
dc.contributor.authorDennison, Ashley R.
dc.contributor.authorSayan, A. Emre
dc.contributor.authorMcDearmid, Jonathan
dc.contributor.authorGreaves, Peter
dc.contributor.authorTulchinsky, Eugene
dc.contributor.authorKriajevska, Marina
dc.date.accessioned2019-12-11T05:46:01Z
dc.date.available2019-12-11T05:46:01Z
dc.date.issued2019-07-02
dc.descriptionhttps://www.nature.com/articles/s41416-019-0483-9#Abs1en_US
dc.description.abstractBackground S100 proteins have been implicated in various aspects of cancer, including epithelial-mesenchymal transitions (EMT), invasion and metastasis, and also in inflammatory disorders. Here we examined the impact of individual members of this family on the invasion of pancreatic ductal adenocarcinoma (PDAC) cells, and their regulation by EMT and inflammation. Methods Invasion of PDAC cells was analysed in zebrafish embryo xenografts and in transwell invasion assays. Expression and regulation of S100 proteins was studied in vitro by immunoblotting, quantitative PCR and immunofluorescence, and in pancreatic lesions by immunohistochemistry. Results Whereas the expression of most S100 proteins is characteristic for epithelial PDAC cell lines, S100A4 and S100A6 are strongly expressed in mesenchymal cells and upregulated by ZEB1. S100A4/A6 and epithelial protein S100A14 respectively promote and represses cell invasion. IL-6/11-STAT3 pathway stimulates expression of most S100 proteins. ZEB1 synergises with IL-6/11-STAT3 to upregulate S100A4/A6, but nullifies the effect of inflammation on S100A14 expression. Conclusion EMT/ZEB1 and IL-6/11-STAT3 signalling act independently and congregate to establish the expression pattern of S100 proteins, which drives invasion. Although ZEB1 regulates expression of S100 family members, these effects are masked by IL-6/11-STAT3 signalling, and S100 proteins cannot be considered as bona fide EMT markers in PDAC.en_US
dc.identifier.citationAl-Ismaeel, Q., Neal, C. P., Al-Mahmoodi, H., Almutairi, Z., Al-Shamarti, I., Straatman, K., … Kriajevska, M. (2019). ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins. British Journal of Cancer, 121(1), 65–75. https://doi.org/10.1038/s41416-019-0483-9en_US
dc.identifier.other000473525700009
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/4356
dc.language.isoenen_US
dc.publisherNATURE PUBLISHING GROUPen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectpancreatic ductal adenocarcinomaen_US
dc.subjectPDACen_US
dc.subjectZEB1en_US
dc.subjectIL-6/11-STAT3en_US
dc.subjectpancreatic cancer cellsen_US
dc.subjectS100 proteinsen_US
dc.titleZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteinsen_US
dc.typeArticleen_US
workflow.import.sourcescience

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