Investigating the Suitability of Mare’s Milk-Derived Exosomes as Potential Drug Carriers.

Simple item page
dc.contributor.authorSergazy, S.
dc.contributor.authorZhetkenev, S
dc.contributor.authorShulgau, Z.
dc.contributor.authorChulenbayeva, L.
dc.contributor.authorKamyshanskiy, Y.
dc.contributor.authorNurgaziyev, M.
dc.contributor.authorNurgozhina, A.
dc.contributor.authorMukhanbetzhanova, Z.
dc.contributor.authorBerikkhanova, K.
dc.contributor.authorGulyayev, A.
dc.contributor.authorAljofan M.
dc.date.accessioned2024-10-31T07:24:32Z
dc.date.available2024-10-31T07:24:32Z
dc.date.issued2024
dc.description.abstractExosomes are cell-derived, membrane-surrounded particles that deliver bioactive molecules to various cells. Due to their small size, low immunogenicity, extended blood circulation, and involvement in cellular communication, they hold potential as effective drug carriers. Exosomes are present in various biological fluids, including mare’s milk, a traditional drink in Central Asia. This study aims to compare exosome isolation methodologies and determine the stability of mare’s milk-derived exosomes as potential therapeutic carriers. Three extraction methods—immunoprecipitation, size exclusion chromatography, and total exosome isolation—were compared in terms of exosome characteristics, purity, and content. The isolated exosomes were then loaded with quercetin, and their ability to increase its bioavailability was tested in vitro and in vivo. Total exosome isolation was identified as the most efficient method for producing high-quality exosomes. These exosomes were loaded with quercetin and compared to free quercetin and exosomes alone. Exosomes loaded with 80 µM quercetin significantly restored β-galactosidase activity and cellular viability in doxorubicin-treated cells, exhibiting similar potency to 160 µM free quercetin. In aged model animals, treatment with quercetin-loaded exosomes resulted in significantly less acute and subacute damage to the myocardium, kidneys, and liver compared to untreated control animals. This study provides a proof-of-concept that mare’s milk-derived exosomes can be effectively absorbed by cells and animal tissues, supporting their potential use as drug carriers.
dc.identifier.citationSergazy, S., Zhetkenev, S., Shulgau, Z., Chulenbayeva, L., Kamyshanskiy, Y., Nurgaziyev, M., Nurgozhina, A., Mukhanbetzhanova, Z., Berikkhanova, K., Gulyayev, A., & Aljofan, M. (2024). Investigating the suitability of mare’s Milk-Derived exosomes as potential drug carriers. Biomolecules, 14(10), 1247, MDPI. https://doi.org/10.3390/biom14101247
dc.identifier.otherdoi.org/10.3390/biom14101247
dc.identifier.urihttps://nur.nu.edu.kz/handle/123456789/8288
dc.language.isoen
dc.publisherMDPI
dc.rightsCC0 1.0 Universalen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/
dc.subjectexosomes
dc.subjectextracellular vesicles
dc.subjectquercetin
dc.subjectaging
dc.subjectROS
dc.titleInvestigating the Suitability of Mare’s Milk-Derived Exosomes as Potential Drug Carriers.
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
biomolecules-14-01247 (1).pdf
Size:
4.37 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
6.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles