SCREENING A COLLECTION OF LOCAL AND FOREIGN VARIETIES OF SOLANUM LYCOPERSICUM L. IN KAZAKHSTAN FOR GENETIC MARKERS OF RESISTANCE AGAINST THREE TOMATO VIRUSES
Loading...
Date
2022
Authors
Pozharskiy, Alexandr
Kostyukova, Valeriya
Taskuzhina, Aisha
Nizamdinova, Gulnaz
Kisselyova, Nina
Kalendar, Ruslan
Karimov, Nurlybek
Gritsenko, Dilyara
Journal Title
Journal ISSN
Volume Title
Publisher
Heliyon
Abstract
The tomato is one of the most important vegetable crops. The successful development of tomato cultures in
Kazakhstan depends on the implementation of intensive agricultural methods, including breeding and selecting
for new tomato varieties resistant to plant pathogens. Common tomato viruses, although not detected in our
country to date, may potentially have a deleterious impact on agriculture if allowed to spread. The implementation
of tomato breeding programs based on molecular markers of resistance is therefore an important
preventive measure for protecting the agriculture and food safety of Kazakhstan. In the present work, we used
nine molecular markers associated with resistance to three tomato viruses, i.e., tomato mosaic virus (ToMV),
tomato spot wilt virus (TSWV), and tomato yellow leaf curl virus (TYLCV), to test the local breeding collection for
the presence of genetic resistance factors. Two tomato varieties, ‘Zhiraf’ (Russia) and ‘Sunnik’ (Armenia), were
revealed to possess the resistant allele marker PrRuG86-151 against ToMV; three hybrid forms had the same allele
in the heterozygous state. One hybrid, based on the ‘Mirsini’ F1 variety from the Netherlands, had resistance to
TSWV, which was confirmed by four markers: NCSw003, NCSw007, NCSw011, and NCSw012. Two cultivars,
‘Nicola’ and ‘Malinovyi Slon’ (Russia), and the local hybrid based on ‘Yarkiy Rumyanets’ had two to three
resistant alleles of markers based on locus Tm-3 of resistance to TYLCV. The obtained results have demonstrated
that the collection of tomato varieties involved in breeding programs in Kazakhstan lacks well-known genetic
resistance factors to the considered tomato viruses. Thus, the prospective breeding programs require introduction
of known resistant genetic resources to establish resistance to viruses using marker-assisted selection.
Description
Keywords
Type of access: Open Access, Tomato, Tomato mosaic virus, Tomato spot wilt virus, Tomato yellow curl leaf virus, SCAR, CAPS
Citation
Pozharskiy, A., Kostyukova, V., Taskuzhina, A., Nizamdinova, G., Kisselyova, N., Kalendar, R., Karimov, N., & Gritsenko, D. (2022). Screening a collection of local and foreign varieties of Solanum lycopersicum L. in Kazakhstan for genetic markers of resistance against three tomato viruses. Heliyon, 8(8), e10095. https://doi.org/10.1016/j.heliyon.2022.e10095