Lactoferrin Is an Allosteric Enhancer of the Proteolytic Activity of Cathepsin G

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dc.contributor.authorEipper, Steffen
dc.contributor.authorSteiner, Robin
dc.contributor.authorLesner, Adam
dc.contributor.authorSienczyk, Marcin
dc.contributor.authorPalesch, David
dc.contributor.authorHalatsch, Marc- Eric
dc.contributor.authorZaczynska, Ewa
dc.contributor.authorHeim, Christopher
dc.contributor.authorHartmann, Marcus D.
dc.contributor.authorZimecki, Michal
dc.contributor.authorRainer Wirtz, Christian
dc.contributor.authorBurster, Timo
dc.date.accessioned2018-08-20T09:06:59Z
dc.date.available2018-08-20T09:06:59Z
dc.date.issued2016-03-17
dc.description.abstractProtease-mediated degradation of proteins is critical in a plethora of physiological processes. Neutrophils secrete serine proteases including cathepsin G (CatG), neutrophile elastase (NE), and proteinase 3 (PR3) together with lactoferrin (LF) as a first cellular immune response against pathogens. Here, we demonstrate that LF increases the catalytic activity of CatG at physiological concentration, with its highest enhancing capacity under acidic (pH 5.0) conditions, and broadens the substrate selectivity of CatG. On a functional level, the enzymatic activity of CatG was increased in the presence of LF in granulocytederived supernatant. Furthermore, LF enhanced CatG-induced activation of platelets as determined by cell surface expression of CD62P. Consequently, LF-mediated enhancement of CatG activity might promote innate immunity during acute inflammation.en_US
dc.identifier.citationEipper S, Steiner R, Lesner A, Sienczyk M, Palesch D, Halatsch M-E, et al. (2016) Lactoferrin Is an Allosteric Enhancer of the Proteolytic Activity of Cathepsin G. PLoS ONE 11(3): e0151509. doi:10.1371/journal.pone.0151509en_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3389
dc.language.isoenen_US
dc.publisherPLOS ONEen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectlactoferrinen_US
dc.subjectcathepsin Gen_US
dc.titleLactoferrin Is an Allosteric Enhancer of the Proteolytic Activity of Cathepsin Gen_US
dc.typeArticleen_US
workflow.import.sourcescience

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