STRUCTURAL CHANGES IN THE MICROBIOME IN RATS WITH DSS-INDUCED COLITIS
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Date
2020
Authors
Kozhakhmetov, S.
Babenko, D.
Tuyakova, A.
Nurgaziyev, M.
Nurgozhina, A.
Muhanbetganov, N.
Chulenbayeva, L.
Sergazy, S.
Gulyayev, A.
Kushugulova, A.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
Microflora plays a critical role in the initiation and development of inflammatory bowel disease (IBD).
There are many facts accumulated about the effect of intestinal dysbiosis on the pathogenesis of Crohn’s
disease and ulcerative colitis (UC). A biological preparation based on intestinal bacteria can modulate
the intestinal normoflora in IBD. We evaluated the effect of the biological drug on rat intestinal microbiome
in DSS-induced colitis.Rats were divided into 4 groups: without a colitis model (n = 7); with a colitis
model received (n=6); experimental group animals (with a colitis model) received a 10% DSS solution
for 7 days, and biology product intragastrically at a dose of 500 mg/kg body weight once per day for 7
days (n = 7); comparison group animals (with a colitis model) received a 10% DSS solution for 7 days and
as a treatment the 5-ASA (5-aminosalicylic acid) intragastrically at a dose of 100 mg / kg of animal body
weight once for 7 days (n = 6). In our work, we found a decrease in bactericides after 7 days of 10% DSS.
An increased in the biodiversity index in the control group is associated with an increase in the following
genera: Murimonas, Victivallales, Anaerofustis and etc. Wang W. et. al. shows a decrease in the number
of Lactobacillus and Bifidobacteria were significantly reduced in DSS-induced colitis. In our study, the
use of biological product show to the partial restoration of certain types of intestinal bacteria such as
gram-positive Clostridium XVIII, Faecalibacterium, gram-negative Microbacter, Phascolarctobacterium
producing SCFAs, etc.
Description
Keywords
microbiome, colitis, rats, Research Subject Categories::MEDICINE