Analysis of GCIP Function in Rheumatoid Arthritis

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dc.contributor.authorNessipbekova, Assem
dc.date.accessioned2024-05-13T10:35:08Z
dc.date.available2024-05-13T10:35:08Z
dc.date.issued2024-04-29
dc.description.abstractThe global burden of rheumatoid arthritis (RA) is increasing for the ageing population. The disease is characterized by progressive joint inflammation and tissue destruction. One of the key players in the pathogenesis of RA is synovial fibroblasts (SF), which invade and destroy the cartilage in the joints. However, the exact molecular mechanism which explains their abnormal outgrowth is yet to be elucidated. Grap2 cyclin D interacting protein (GCIP), a protein related to Id proteins, plays a role in cell cycle progression and gene expression by blocking interactions between (CREB) binding protein (CBP) and RNA polymerase II complexes. A recent study has shown that Grap2 cyclin D interacting protein (GCIP) has been downregulated in the synovial fibroblasts in rheumatoid arthritis patients, suggesting its involvement in disease progression. The knockdown of the GCIP protein resulted in the increased growth of synovial fibroblasts, consequently leading to the upregulation of Cyclin D1, a crucial cell cycle and cell proliferation player regulated by the CREB. This thesis aimed to explore the role of GCIP in the pathogenesis of rheumatoid arthritis by establishing a mouse model of rheumatoid arthritis and testing how GCIP knockout affects the proliferation of synovial fibroblast during RA. Two cohorts of WT and GCIP KO C57BL/6 mice were utilized to induce RA with 2 different protocols. The protocol 2 resulted in a more pronounced disease phenotype in males. Female mice from both showed no visible signs of RA. The role of GCIP on the proliferation of the synovial fibroblasts was not established necessitating a larger cohort size and choosing male mice as a subject.en_US
dc.identifier.citationNessipbekova, Assem. (2024) Analysis of GCIP Function in Rheumatoid Arthritis. Nazarbayev University School of Medicineen_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/7658
dc.language.isoenen_US
dc.publisherNazarbayev University School of Medicineen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectType of access: Restricteden_US
dc.subjectrheumatoid arthritisen_US
dc.subjectsynovial fibroblastsen_US
dc.subjectGCIPen_US
dc.titleAnalysis of GCIP Function in Rheumatoid Arthritisen_US
dc.typeMaster's thesisen_US
workflow.import.sourcescience

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