TRIM28 IS A NOVEL REGULATOR OF CD133 EXPRESSION ASSOCIATED WITH CANCER STEM CELL PHENOTYPE

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dc.contributor.authorKim, Yan S.
dc.contributor.authorPotashnikova, Daria M.
dc.contributor.authorGisina, Alisa M.
dc.contributor.authorKholodenko, Irina V.
dc.contributor.authorKopylov, Arthur T.
dc.contributor.authorTikhonova, Olga V.
dc.contributor.authorKurbatov, Leonid K.
dc.contributor.authorSaidova, Aleena A.
dc.contributor.authorTvorogova, Anna V.
dc.contributor.authorKholodenko, Roman V.
dc.contributor.authorBelousov, Pavel V.
dc.contributor.authorVorobjev, Ivan A.
dc.contributor.authorZgoda, Victor G.
dc.contributor.authorYarygin, Konstantin N.
dc.contributor.authorLupatov, Alexey Yu.
dc.date.accessioned2023-04-20T04:30:55Z
dc.date.available2023-04-20T04:30:55Z
dc.date.issued2022
dc.description.abstractCD133 is an extensively studied marker of the most malignant tumor cell population, designated as cancer stem cells (CSCs). However, the function of this glycoprotein and its involvement in cell regulatory cascades are still poorly understood. Here we show a positive correlation between the level of CD133 plasma membrane expression and the proliferative activity of cells of the Caco-2, HT-29, and HUH7 cancer cell lines. Despite a substantial difference in the proliferative activities of cell populations with different levels of CD133 expression, transcriptomic and proteomic profiling revealed only minor distinctions between them. Nonetheless, a further in silico assessment of the differentially expressed transcripts and proteins revealed 16 proteins that could be involved in the regulation of CD133 expression; these were assigned ranks reflecting the apparent extent of their involvement. Among them, the TRIM28 transcription factor had the highest rank. The prominent role of TRIM28 in CD133 expression modulation was confirmed experimentally in the Caco2 cell line clones: the knockout, though not the knockdown, of the TRIM28 gene downregulated CD133. These results for the first time highlight an important role of the TRIM28 transcription factor in the regulation of CD133-associated cancer cell heterogeneity.en_US
dc.identifier.citationKim, Y. S., Potashnikova, D. M., Gisina, A. M., Kholodenko, I. V., Kopylov, A. T., Tikhonova, O. V., Kurbatov, L. K., Saidova, A. A., Tvorogova, A. V., Kholodenko, R. V., Belousov, P. V., Vorobjev, I. A., Zgoda, V. G., Yarygin, K. N., & Lupatov, A. Y. (2022). TRIM28 Is a Novel Regulator of CD133 Expression Associated with Cancer Stem Cell Phenotype. International Journal of Molecular Sciences, 23(17), 9874. https://doi.org/10.3390/ijms23179874en_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/7033
dc.language.isoenen_US
dc.publisherInternational Journal of Molecular Sciencesen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectType of access: Open Accessen_US
dc.subjectcancer stem cellsen_US
dc.subjectCD133en_US
dc.subjectcell signalingen_US
dc.subjectTRIM28en_US
dc.subjectstemness markersen_US
dc.titleTRIM28 IS A NOVEL REGULATOR OF CD133 EXPRESSION ASSOCIATED WITH CANCER STEM CELL PHENOTYPEen_US
dc.typeArticleen_US
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