ISOLATION OF NATURAL REPRESENTATIVES OF CARP LACTOFLORA TO CREATE PATHOGEN CONTROL AGENTS THAT CAN BE ALTERNATIVE TO ANTIBIOTICS
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Date
2020
Authors
Urazova, M.
Zakarya, K.
Sarmurzina, Z.
Bissenova, G.
Abitayeva, G.
Shevtsov, A.
Tekebayeva, Zh.
Tynybayeva, I.
Abzhalelov, A.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
Currently, the microbiome of commercial fish species is being actively studied. In reviews, mainly based
on metagenome analysis features and formation of intestinal microflora of various fish species of North
America, Asia and Europe have been presented. Results of these researches showed that lactic acid bacteria
(LAB) are common microbial group of intestinal microbiota of fish (Wu et al., 2012; Ye et al., 2014;
Borsodiet al., 2017; Wang et al., 2018). In our research a characteristic of 22lactic acid bacteria isolated
from Common Carp (Cyprinus carpio) intestines were studied. All of them were tested for their ability
to inhibit growth of Aeromonas punctate, Shewanella xiamenensis, Pseudomonas aeruginosa,Pseudomonas
taiwanensis.In sum, 7 isolates with high antagonistic activity were selected. 16S rDNA gene sequencing
identified them as Lactobacillus fermentum(4), Lactobacillus casei/paracasei (2) and Pediococcus pentosaceus
(1). All identified isolates can grow in wide temperature range (10° C to 37° C) and in presence of
bile. Earlier the species of Lactobacillus, Lactococcus, Streptococcus, Enterococcus,Pediococcus and Carnobacteriumgenera
were isolated from carp intestines (Bucio et al., 2006; Wang et al., 2018). We isolated
only strains, belonging to 3 species (Lactobacillus fermentum, Lactobacillus casei/paracasei and Pediococcus
pentosaceus). It is low rate of species diversity. Perhaps this is due to the conditions, when we made isolationof
LAB. It wasmade at the transition period from wintering to active spawning.The climate of Central
and Northern Kazakhstan is severe and it is characterized by a cold winter lasting 5,5-6 months. Long
wintering is a stress for an animal, and its microflora too. During the wintering period, carps do not feed,
the composition of their microflora becomes poorer.The dominance of somespecies among intestinal
LAB during cold period should be due to their specific characteristics, such as resistance to low temperatures
and unfavorable environmental factors.Selective 7 strains could be alternatives to antibiotics for
freshwater aquaculture in Kazakhstan.
Description
Keywords
Research Subject Categories::MEDICINE, лактобактерии, микробиота, карп, аквакультура, пробиотики