Development of a technology for expression of recombinant human erythropoietin in cultured mammalian cells using alphavirus expression system

Abstract

Currently there are no industrial eukaryotic expression systems other than transient expression from plasmids or expression from genes integrated into host genome. Both approaches (use of eukaryotic plasmids or chromosomal integration) suffer from poor scalability and often from poor yields. Although, in laboratory settings, effective means for transducing of cultured cells to express foreign proteins and for high-level transient expression were developed based on viral genomes. We thought to develop a scalable and suitable for industrial application technology for the production of recombinant human erythropoietin (EPO) in mammalian cell cultures using an expression vector based on the genome of RNA virus.