PURIFICATION OF TOMATO BUSHY STUNT VIRUS PARTICLES BY ONE-STEP HYDROXYAPATITE COLUMN CHROMATOGRAPHY

Simple item page
dc.contributor.authorTleukulova, Zh.
dc.contributor.authorStamgaliyeva, Z.
dc.contributor.authorDildabek, A.
dc.contributor.authorMukiyanova, G.
dc.contributor.authorOmarov, R.T.
dc.date.accessioned2022-12-01T08:01:20Z
dc.date.available2022-12-01T08:01:20Z
dc.date.issued2021
dc.description.abstractThe main aim of this work was to develop a time-saving and cost-effective purification method of infectious plant viral nanoparticles. Virions of Tomato bushy stunt virus (TBSV), which is a member of Tombusvirus genus, were purified by one-step Biogel HT Hydroxyapatite (HA) column chromatography. Extracts from Nicotiana benthamiana plants infected with TBSV were directly loaded onto the HA column and eluted by 10 mM sodium phosphate buffer (pH 6.8). A specificity of virions has been confirmed by immunoblotting and electron microscopy. Homogeneity of virions was tested by SDS-PAGE, where only 41 kDa polypeptide bands referring to the capsid protein of TBSV were detected by Coomassie staining. The biological infectious activity of a purified material was demonstrated by observing TBSVspecific symptoms observed in N. benthamiana plants at 7‒10 days of postinoculation (dpi). Moreover, purified virions were used for immunization of the BALb/c mouse to raise primary antibodies against the TBSV virus. Our results show that in low concentrations of sodium phosphate buffer total proteins extracted from infected plants adsorb to HA sorbent, while viral particles do not adsorb to the HA matrix and flow throw column due to Ca2+ ions implicated in TBSV virions’ structure. This highly effective and simple virus purification protocol can also be used for the isolation of other plant virions.en_US
dc.identifier.citationTleukulova, Z., Stamgaliyeva, Z., Dildabek, A., Mukiyanova, G., & Omarov, R. (2021b). Purification of Tomato Bushy Stunt Virus Particles by One-Step Hydroxyapatite Column Chromatography. Eurasian Chemico-Technological Journal, 23(4), 277. https://doi.org/10.18321/ectj1131en_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/6846
dc.language.isoenen_US
dc.publisherEURASIAN CHEMICO-TECHNOLOGICAL JOURNALen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectType of access: Open Accessen_US
dc.subjectVirus particlesen_US
dc.subjectTomato bushy stunt virusen_US
dc.subjectPurificationen_US
dc.subjectHydroxyapatite column chromatographyen_US
dc.titlePURIFICATION OF TOMATO BUSHY STUNT VIRUS PARTICLES BY ONE-STEP HYDROXYAPATITE COLUMN CHROMATOGRAPHYen_US
dc.typeArticleen_US
workflow.import.sourcescience

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1131-Article Text-1811-1-10-20220316.pdf
Size:
763.36 KB
Format:
Adobe Portable Document Format
Description:
article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
6.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles