Engineering of cell membranes with a bisphosphonate-containing polymer using ATRP synthesis for bone targeting

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dc.contributor.authorD'Souza, Sonia
dc.contributor.authorMurata, Hironobu
dc.contributor.authorJose, Moncy V.
dc.contributor.authorAskarova, Sholpan
dc.contributor.authorYantsen, Yuliya
dc.contributor.authorAndersen, Jill D.
dc.contributor.authorEdington, Collin D.J.
dc.contributor.authorClafshenkel, William P.
dc.contributor.authorKoepsel, Richard R.
dc.contributor.authorRussell, Alan J.
dc.creatorSonia, D'Souza
dc.date.accessioned2018-01-04T09:22:10Z
dc.date.available2018-01-04T09:22:10Z
dc.date.issued2014-11-01
dc.description.abstractAbstract The field of polymer-based membrane engineering has expanded since we first demonstrated the reaction of N-hydroxysuccinimide ester-terminated polymers with cells and tissues almost two decades ago. One remaining obstacle, especially for conjugation of polymers to cells, has been that exquisite control over polymer structure and functionality has not been used to influence the behavior of cells. Herein, we describe a multifunctional atom transfer radical polymerization initiator and its use to synthesize water-soluble polymers that are modified with bisphosphonate side chains and then covalently bound to the surface of live cells. The polymers contained between 1.7 and 3.1 bisphosphonates per chain and were shown to bind to hydroxyapatite crystals with kinetics similar to free bisphosphonate binding. We engineered the membranes of both HL-60 cells and mesenchymal stem cells in order to impart polymer-guided bone adhesion properties on the cells. Covalent coupling of the polymer to the non-adherent HL-60 cell line or mesenchymal stem cells was non-toxic by proliferation assays and enhanced the binding of these cells to bone.
dc.identifierDOI:10.1016/j.biomaterials.2014.07.041
dc.identifier.citationSonia D'Souza, Hironobu Murata, Moncy V. Jose, Sholpan Askarova, Yuliya Yantsen, Jill D. Andersen, Collin D.J. Edington, William P. Clafshenkel, Richard R. Koepsel, Alan J. Russell, Engineering of cell membranes with a bisphosphonate-containing polymer using ATRP synthesis for bone targeting, In Biomaterials, Volume 35, Issue 35, 2014, Pages 9447-9458
dc.identifier.issn01429612
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0142961214008503
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3098
dc.relation.ispartofBiomaterials
dc.rights.licenseCopyright © 2014 Elsevier Ltd. All rights reserved.
dc.subjectMembrane engineering
dc.subjectATRP
dc.subjectBone targeting polymer
dc.subjectCell reactive polymers
dc.titleEngineering of cell membranes with a bisphosphonate-containing polymer using ATRP synthesis for bone targeting
dc.typeArticle
dcterms.publisherBiomaterials
elsevier.aggregationtypeJournal
elsevier.coverdate2014-11-01
elsevier.coverdisplaydateNovember 2014
elsevier.endingpage9458
elsevier.identifier.doi10.1016/j.biomaterials.2014.07.041
elsevier.identifier.eid1-s2.0-S0142961214008503
elsevier.identifier.piiS0142-9612(14)00850-3
elsevier.identifier.pubmedid25150889
elsevier.identifier.scopusid84961288380
elsevier.issue.identifier35
elsevier.openaccess0
elsevier.openaccessarticlefalse
elsevier.openarchivearticlefalse
elsevier.startingpage9447
elsevier.teaserThe field of polymer-based membrane engineering has expanded since we first demonstrated the reaction of N-hydroxysuccinimide ester-terminated polymers with cells and tissues almost two decades ago....
elsevier.volume35

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Research Institute of Bioengineering, Regenerative Medicine and Neurophysiology