The effects of antiviral treatment on breast cancer cell line

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dc.contributor.authorMektepbayeva, Damel
dc.contributor.authorShaimerdenova, Madina
dc.contributor.authorKarapina, Orynbassar
dc.contributor.authorAlibek, Kenneth
dc.contributor.authorAkilbekova, Dana
dc.date.accessioned2018-11-01T10:40:19Z
dc.date.available2018-11-01T10:40:19Z
dc.date.issued2017
dc.description.abstractBackground: Recent studies have revealed the positive antiproliferative and cytotoxic effects of antiviral agents in cancer treatment. The real effect of adjuvant antiviral therapy is still controversial due to the lack of studies in biochemical mechanisms. Here, we studied the effect of the antiviral agent acyclovir on morphometric and migratory features of the MCF7 breast cancer cell line. Molecular levels of various proteins have also been examined. Methods: To evaluate and assess the effect of antiviral treatment on morphometric, migratory and other cellular characteristics of MCF7 breast cancer cells, the following experiments were performed: (i) MTT assay to measure the viability of MCF7 cells; (ii) Colony formation ability by soft agar assay; (iii) Morphometric characterization by immunofluorescent analysis using confocal microscopy; (iv) wound healing and transwell membrane assays to evaluate migration and invasion capacity of the cells; (v) ELISA colorimetric assays to assess expression levels of caspase-3, E-cadherin and enzymatic activity of aldehyde dehydrogenase (ALDH). Results: We demonstrate the suppressive effect of acyclovir on breast cancer cells. Acyclovir treatment decreases the growth and the proliferation rate of cells and correlates with the upregulated levels of apoptosis associated cytokine Caspase-3. Moreover, acyclovir inhibits colony formation ability and cell invasion capacity of the cancer cells while enhancing the expression of E-cadherin protein in MCF7 cells. Breast cancer cells are characterized by high ALDH activity and associated with upregulated proliferation and invasion. According to this study, acyclovir downregulates ALDH activity in MCF7 cells. Conclusions: These results are encouraging and demonstrate the possibility of partial suppression of cancer cell proliferation using an antiviral agent. Acyclovir antiviral agents have a great potential as an adjuvant therapy in the cancer treatment. However, more research is necessary to identify relevant biochemical mechanisms by which acyclovir induces a potent anti-cancer effect.en_US
dc.identifier.citationMadina Shaimerdenova, Orynbassar Karapina, Damel Mektepbayeva, Kenneth Alibek and Dana Akilbekova. The effects of antiviral treatment on breast cancer cell line. 2017. Infectious Agents and Canceren_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3596
dc.language.isoenen_US
dc.publisherInfectious Agents and Canceren_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectMCF7 breast cancer cell lineen_US
dc.subjectAcycloviren_US
dc.subjectAntiproliferative effecten_US
dc.subjectALDH activityen_US
dc.titleThe effects of antiviral treatment on breast cancer cell lineen_US
dc.typeArticleen_US
workflow.import.sourcescience

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