Histological and biochemical analysis of DNA damage after BNCT in rat model

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dc.contributor.authorMasutani, Mitsuko
dc.contributor.authorBaiseitov, Diaz
dc.contributor.authorItoh, Tasuku
dc.contributor.authorHirai, Takahisa
dc.contributor.authorBerikkhanova, Kulzhan
dc.contributor.authorMurakami, Yasufumi
dc.contributor.authorZhumadilov, Zhaxybay
dc.contributor.authorImahori, Yoshio
dc.contributor.authorHoshi, Masaharu
dc.contributor.authorItami, Jun
dc.creatorMitsuko, Masutani
dc.date.accessioned2018-01-04T08:57:35Z
dc.date.available2018-01-04T08:57:35Z
dc.date.issued2014-06-01
dc.description.abstractAbstract To understand the mechanism of tumor cell death induced by boron neutron capture therapy (BNCT) and to optimize BNCT condition, we used rat tumor graft models and histological and biochemical analyses were carried out focusing on DNA damage response. Rat lymphosarcoma cells were grafted subcutaneously into male Wister rats. The rats with developed tumors were then treated with neutron beam irradiation 45min after injection of 330mg/kg bodyweight boronophenylalanine (10BPA) (+BPA) or saline control (–BPA). BNCT was carried out in the National Nuclear Center of the Republic of Kazakhstan (neutron flux: 1×109nvt/s, fluence: 6×1011nvt) with the presence of background γ-irradiation of 33Gy. 6 and 20h after BNCT treatment, tumors were resected, fixed and subjected to immunohistochemistry and biochemical analyses. Immunostaining of nuclei showed that double strand break (DSB) marker gamma H2AX staining was high in 20h/+BPA sample but not in 20h/–BPA samples. Poly(ADP-ribose), DSB and single strand break markers of DNA, also demonstrated this tendency. These two markers were observed at low levels in unirradiated tissues or 6h after BNCT either under −BPA and +BPA conditions. HMGB1 level increased in 6h/+BPA but not in 6h/−BPA or 20h/+BPA samples. The persistent staining of γH2AX and poly(ADP-ribose) in +BPA group suggests accumulated DSB damage after BNCT. The early HMGB1 upregulation and γH2AX and poly(ADP-ribose) observed later might be the markers for monitoring the DNA damage induced by BNCT.en_US
dc.identifierDOI:10.1016/j.apradiso.2014.03.003
dc.identifier.citationMitsuko Masutani, Diaz Baiseitov, Tasuku Itoh, Takahisa Hirai, Kulzhan Berikkhanova, Yasufumi Murakami, Zhaxybay Zhumadilov, Yoshio Imahori, Masaharu Hoshi, Jun Itami, Histological and biochemical analysis of DNA damage after BNCT in rat model, In Applied Radiation and Isotopes, Volume 88, 2014, Pages 104-108en_US
dc.identifier.issn09698043
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0969804314000803
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3094
dc.language.isoenen_US
dc.publisherApplied Radiation and Isotopesen_US
dc.relation.ispartofApplied Radiation and Isotopes
dc.rights.licenseCopyright © 2014 Elsevier Ltd. All rights reserved.
dc.subjectBNCTen_US
dc.subjectDNA damage responseen_US
dc.subjectBPAen_US
dc.subjectγH2AXen_US
dc.subjectPARen_US
dc.subjectHMGB1en_US
dc.titleHistological and biochemical analysis of DNA damage after BNCT in rat modelen_US
dc.typeArticleen_US
elsevier.aggregationtypeJournal
elsevier.coverdate2014-06-01
elsevier.coverdisplaydateJune 2014
elsevier.endingpage108
elsevier.identifier.doi10.1016/j.apradiso.2014.03.003
elsevier.identifier.eid1-s2.0-S0969804314000803
elsevier.identifier.piiS0969-8043(14)00080-3
elsevier.identifier.pubmedid24690552
elsevier.identifier.scopusid84901836830
elsevier.issue.name15th International Congress on Neutron Capture Therapy Impact of a new radiotherapy against cancer
elsevier.openaccess0
elsevier.openaccessarticlefalse
elsevier.openarchivearticlefalse
elsevier.startingpage104
elsevier.teaserTo understand the mechanism of tumor cell death induced by boron neutron capture therapy (BNCT) and to optimize BNCT condition, we used rat tumor graft models and histological and biochemical analyses...
elsevier.volume88
workflow.import.sourcescience

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