DYNAMIC CENTRIOLAR LOCALIZATION OF POLO AND CENTROBIN IN EARLY MITOSIS PRIMES CENTROSOME ASYMMETRY
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Date
2020-08-06
Authors
Gallaud, Emmanuel
Nair, Anjana Ramdas
Horsley, Nicole
Monnard, Arnaud
Singh, Priyanka
Pham, Tri Thanh
Garcia, David Salvador
Ferrand, Alexia
Cabernard, Clemens
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Abstract
Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain
an older “mother” and a younger “daughter” centriole. Stem cells either inherit the mother or
daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry
and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the
mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the
daughter centriole during mitosis, thereby generating molecularly distinct mother and
daughter centrioles before interphase. Cnb’s asymmetric localization, potentially involving a
direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas
Polo’s daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic
protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle
orientation.
Description
Keywords
Centrioles, Centrosomes, Neuroblasts, Cell cycle and cell division, Mitosis, Green fluorescent protein, Microtubules, Prophase, Research Subject Categories::NATURAL SCIENCES::Biology, Type of access: Open Access
Citation
Gallaud, E., Ramdas Nair, A., Horsley, N., Monnard, A., Singh, P., Pham, T. T., Salvador Garcia, D., Ferrand, A., & Cabernard, C. (2020). Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLOS Biology, 18(8), e3000762. https://doi.org/10.1371/journal.pbio.3000762