Brillouin spectroscopy and radiography for assessment of viscoelastic and regenerative properties of mammalian bones
Loading...
Date
2018-09-27
Authors
Akilbekova, Dana
Ogay, Vyacheslav
Yakupov, Talgat
Sarsenova, Madina
Umbayev, Bauyrzhan
Nurakhmetov, Asset
Tazhin, Kairat
Yakovlev, Vladislav V.
Utegulov, Zhandos N.
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Biomedical Optics
Abstract
Biomechanical properties of mammalian bones, such as strength, toughness, and plasticity, are
essential for understanding how microscopic-scale mechanical features can link to macroscale bones’ strength
and fracture resistance. We employ Brillouin light scattering (BLS) microspectroscopy for local assessment of
elastic properties of bones under compression and the efficacy of the tissue engineering approach based on
heparin-conjugated fibrin (HCF) hydrogels, bone morphogenic proteins, and osteogenic stem cells in the regeneration
of the bone tissues. BLS is noninvasive and label-free modality for probing viscoelastic properties of
tissues that can give information on structure-function properties of normal and pathological tissues. Results
showed that MCS and BPMs are critically important for regeneration of elastic and viscous properties, respectively,
HCF gels containing combination of all factors had the best effect with complete defect regeneration at
week nine after the implantation of bone grafts and that the bones with fully consolidated fractures have higher
values of elastic moduli compared with defective bones
Description
Keywords
Brillouin light scattering, bones, critical-sized defect, biomechanical properties, elastic, viscous, heparin-conjugated fibrin gel, bone morphogenic proteins, mesenchymal stem cells
Citation
Dana Akilbekova, Vyacheslav Ogay, Talgat Yakupov, Madina Sarsenova, Bauyrzhan Umbayev, Asset Nurakhmetov, Kairat Tazhin, Vladislav V. Yakovlev, Zhandos N. Utegulov, “Brillouin spectroscopy and radiography for assessment of viscoelastic and regenerative properties of mammalian bones,” J. Biomed. Opt. 23(9), 097004 (2018), doi: 10.1117/1.JBO.23.9.097004.