Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

Simple item page
dc.contributor.authorShevtsova, Elena
dc.contributor.authorShevtsov, Alexandr
dc.contributor.authorMukanov, Kasim
dc.contributor.authorFilipenko, Maxim
dc.contributor.authorKamalova, Dinara
dc.contributor.authorSytnik, Igor
dc.contributor.authorSyzdykov, Marat
dc.contributor.authorKuznetsov, Andrey
dc.contributor.authorAkhmetova, Assel
dc.contributor.authorZharova, Mira
dc.contributor.authorKaribaev, Talgat
dc.contributor.authorTarlykov, Pavel
dc.contributor.authorRamanculov, Erlan
dc.date.accessioned2017-11-16T05:26:31Z
dc.date.available2017-11-16T05:26:31Z
dc.date.issued2016-12-01
dc.description.abstractBrucellosis is a major zoonotic infection in Kazakhstan. However, there is limited data on its incidence in humans and animals, and the genetic diversity of prevalent strains is virtually unstudied. Additionally, there is no detailed overview of Kazakhstan brucellosis control and eradication programs. Here, we analyzed brucellosis epidemiological data, and assessed the effectiveness of eradication strategies employed over the past 70 years to counteract this infection. We also conducted multiple loci variable-number tandem repeat analysis (MLVA) of Brucella abortus strains found in Kazakhstan. We analyzed official data on the incidence of animal brucellosis in Kazakhstan. The records span more than 70 years of antibrucellosis campaigns, and contain a brief description of the applied control strategies, their effectiveness, and their impact on the incidence in humans. The MLVA-16 method was used to type 94 strains of B. abortus and serial passages of B. abortus 82, a strain used in vaccines. MLVA-8 and MLVA-11 analyses clustered strains into a total of four and sevengenotypes, respectively; it is the first time that four of these genotypes have been described. MLVA-16 analysis divided strains into 28 distinct genotypes having genetic similarity coefficient that varies from 60 to100% and a Hunter & Gaston diversity index of 0.871. MST analysis reconstruction revealed clustering into "Kazakhstani-Chinese (Central Asian)", "European" and "American" lines. Detection of multiple genotypes in a single outbreak confirms that poorly controlled trade of livestock plays a crucial role in the spread of infection. Notably, the MLVA-16 profile of the B. abortus 82 strain was unique and did not change during 33 serial passages. MLVA genotyping may thus be useful for epidemiological monitoring of brucellosis, and for tracking the source(s) of infection. We suggest that countrywide application of MLVA genotyping would improve the control of brucellosis in Kazakhstan.ru_RU
dc.identifier.citationShevtsova Elena et al.(>12), 2016(December 1), Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan, Plos Oneru_RU
dc.identifier.uriDOI:10.1371/journal.pone.0167496
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/2821
dc.language.isoenru_RU
dc.publisherPlos Oneru_RU
dc.rightsOpen Access - the content is available to the general publicru_RU
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectbrucellosisru_RU
dc.subjectzoonoticru_RU
dc.subjectKazakhstanru_RU
dc.subjectMLVAru_RU
dc.subjectMLVA-16ru_RU
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Biologyru_RU
dc.titleEpidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstanru_RU
dc.typeArticleru_RU

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Epidemiology.pdf
Size:
2.91 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles