Study the Effect of Different Renewable Carbon Sources on the Succinic Acid Biosynthesis by Optimization Culture Composition using 4-Liter Scale Bioreactor

Abstract

Background: The succinic acid demand accelerated through the years. Thus, the need to improve acid production economically is aggravated. The fermentation process by succinate bacteria showed promising acid production. However, due to different bacteria characteristics, mediums composition and operating conditions, a fixed quantity of succinic acid production cannot be determined or generalized. Recently, raising interest in examining cheap mediums in the fermentation process brought attention to utilizing different raw materials. Nevertheless, its influence on production is not fully comprehended. Methods: This research aims to develop an evaluation of three succinate bacteria on succinic acid production. Also, it aims to demonstrate a comparative analysis of the Succinic acid production by three raw carbon sources (Corn fiber, Cane molasses and orange peel). The flask batch method and the 4-L Bioreactor were employed in the evaluation. Result: The results of the study showed that the Actinobacillus succinogenes is the best succinate bacteria to provide commercial Succinic acid. The presence of 10 g of glucose was satisfactory in improving the fermentation among all bacteria. The highest Succinic acid production was 12.8 g/L provided by the corn fiber. The lowest succinic acid production was 18% less than the glucose medium that the orange peel gave. The results revealed a significant impact of the raw material composition on the production outcomes. At the bioreactor, the cells exhibited an exponential phase with rate growth of 0.175 [g/L.h] and the maximum Succinic acid produced was 25 g/L.