GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES AMONG RECURRENT CASES OF TUBERCULOSIS IN KAZAKHSTAN
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Date
2020
Authors
Akhmetova, A.
Akilzhanova, A.
Toksanbaeva, B.
Bismilda, V.
Chingissova, L.
Kozhamkulov, U.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDEā; National Laboratory Astana
Abstract
Introduction: Tuberculosis still remains one of the major health problems in Kazakhstan. According
to the World Health Organization data (2018), Kazakhstan is on the list of 30 countries with high rates
of multidrug resistant tuberculosis in the world. MIRU-VNTR analysis is widely used genotyping method
of M. tuberculosis. The aimof this work is to characterize biological diversity of M. tuberculosis clinical
isolates among recurrent cases of tuberculosis in Kazakhstan.
Materials and methods: 95 clinical isolates of M. tuberculosis were collected from different regions
of Kazakhstan among recurrent cases of tuberculosis. Genotyping of all isolates was performed by 15
MIRU-VNTR approach. PCR products were visualized on 2% agarose gel stained with ethidium bromide.
Quantity 1(BioRad) program was used to determine the PCR fragment size and calculate the number of
tandem repeats in each MIRU-VNTR locus. 15-digit allelic profiles of all clinical isolates were uploaded to
MIRU-VNTRplus web application to identify M. tuberculosis families. Phylogenetic tree was built using
UPGMA (Unweighted pair group method with arithmetic mean) algorithm.
Results: 40 genotypes were detected based on15 MIRU-VNTR genotyping results. 31(32,6%) genotypes
were unique and found only in one isolate in the collection. The rest 64 (67,4%) M. tuberculosis clinical
isolates were clustered into 9 clusters which included from 2to 34 isolates in one cluster. 87,4% of all
clinical isolates were belonged to Beijing family. The second meaningful family that is found among the
isolates was LAM family (10,5%). URAL and CAS families were identified in the rest 2,1% cases.
Conclusion: Thus, 15 MIRU-VNTR typing of 95 clinical isolates of M. tuberculosisshowed that Beijing
family strains that are associated with drug resistance prevail among recurrent cases of tuberculosis in
Kazakhstan (87,4%).
Description
Keywords
genetics, tuberculosis, MIRU-VNTR analysis, Research Subject Categories::MEDICINE