Using of alphavirus capsid mutant as a vector for the production of recombinant proteins

Abstract

Recombinant DNA technology is used in various ways in a healthcare. By the genetic engineering techniques recombinant protein with pharmaceutical activity aka biopharmaceuticals are produced. The most of approved drugs are produced by mammalian cells. Because, in order to function properly in human organism, recombinant proteins have to pass through glycosilation which can be performed in mammalian cells. In this research we investigated alphaviral vector based on Venezuelan encephalitis virus (VEEV) as possible gene delivery system for mammalian cells transduction.