DEVELOPMENT AND VALIDATION OF HYBRID BRILLOUIN-RAMAN SPECTROSCOPY FOR NONCONTACT ASSESSMENT OF MECHANO-CHEMICAL PROPERTIES OF URINE PROTEINS AS BIOMARKERS OF KIDNEY DISEASES

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dc.contributor.authorGaipov, Abduzhappar
dc.contributor.authorUtegulov, Zhandos N.
dc.contributor.authorBukasov, Rostislav
dc.contributor.authorTurebekov, Duman
dc.contributor.authorTarlykov, Pavel
dc.contributor.authorMarkhametova, Zhannur
dc.contributor.authorNurekeyev, Zhangatay
dc.contributor.authorKunushpayeva, Zhanar
dc.contributor.authorSultangaziyev, Alisher
dc.date.accessioned2021-07-02T03:23:04Z
dc.date.available2021-07-02T03:23:04Z
dc.date.issued2020-06-15
dc.description.abstractBackground Proteinuria is a major marker of chronic kidney disease (CKD) progression and the predictor of cardiovascular mortality. The rapid development of renal failure is expected in those patients who have higher level of proteinuria however, some patients may have slow decline of renal function despite lower level of urinary protein excretion. The different mechanical (visco-elastic) and chemical properties, as well as the proteome profiles of urinary proteins might explain their tubular toxicity mechanism. Brillouin light scattering (BLS) and surface enhanced Raman scattering (SERS) spectroscopies are non-contact, laser optical-based techniques providing visco-elastic and chemical property information of probed human biofluids. We proposed to study and compare these properties of urinary proteins using BLS and SERS spectroscopies in nephrotic patient and validate hybrid BLS-SERS spectroscopy in diagnostic of urinary proteins as well as their profiling. The project ultimately aims for the development of an optical spectroscopic sensor for rapid, non-contact monitoring of urine samples from patients in clinical settings. Methods BLS and SERS spectroscopies will be used for non-contact assessment of urinary proteins in proteinuric patients and healthy subjects and will be cross-validated by Liquid Chromatography-Mass Spectrometry (LC-MS). Participants will be followed-up during the 1 year and all adverse events such as exacerbation of proteinuria, progression of CKD, complications of nephrotic syndrome, disease relapse rate and inefficacy of treatment regimen will be registered referencing incident dates. Associations between urinary protein profiles (obtained from BLS and SERS as well as LC-MS) and adverse outcomes will be evaluated to identify most unfavored protein profiles. Discussion This prospective study is focused on the development of non-contact hybrid BLS - SERS sensing tool and its clinical deployment for diagnosis and prognosis of proteinuria. We will identify the most important types of urine proteins based on their visco-elasticity, amino-acid profile and molecular weight responsible for the most severe cases of proteinuria and progressive renal function decline. We will aim for the developed hybrid BLS - SERS sensor, as a new diagnostic & prognostic tool, to be transferred to other biomedical applications. Trial registration The trial has been approved by ClinicalTrials.gov (Trial registration ID NCT04311684). The date of registration was March 17, 2020.en_US
dc.identifier.citationGaipov, A., Utegulov, Z., Bukasov, R., Turebekov, D., Tarlykov, P., Markhametova, Z., Nurekeyev, Z., Kunushpayeva, Z., & Sultangaziyev, A. (2020). Development and validation of hybrid Brillouin-Raman spectroscopy for non-contact assessment of mechano-chemical properties of urine proteins as biomarkers of kidney diseases. BMC Nephrology, 21(1). https://doi.org/10.1186/s12882-020-01890-xen_US
dc.identifier.issn1471-2369
dc.identifier.urihttps://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-020-01890-x
dc.identifier.urihttps://doi.org/10.1186/s12882-020-01890-x
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/5509
dc.language.isoenen_US
dc.publisherBMCen_US
dc.relation.ispartofseriesBMC Nephrology;21, Article number: 229 (2020)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectResearch Subject Categories::MEDICINEen_US
dc.subjectBrillouin light scatteringen_US
dc.subjectType of access: Open Accessen_US
dc.subjectRaman scatteringen_US
dc.subjectSERSen_US
dc.subjectUrine proteomicsen_US
dc.subjectNon-contact diagnosticsen_US
dc.subjectProteinuriaen_US
dc.subjectViscoelasticen_US
dc.subjectChemicalen_US
dc.subjectBiochemicaen_US
dc.titleDEVELOPMENT AND VALIDATION OF HYBRID BRILLOUIN-RAMAN SPECTROSCOPY FOR NONCONTACT ASSESSMENT OF MECHANO-CHEMICAL PROPERTIES OF URINE PROTEINS AS BIOMARKERS OF KIDNEY DISEASESen_US
dc.typeArticleen_US
workflow.import.sourcescience

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