ACTIVITY-BASED PROBES TO UTILIZE THE PROTEOLYTIC ACTIVITY OF CATHEPSIN G IN BIOLOGICAL SAMPLES
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Date
2021-02-25
Authors
Burster, Timo
Gärtner, Fabian
Knippschild, Uwe
Zhanapiya, Anuar
Journal Title
Journal ISSN
Volume Title
Publisher
Front. Chem.
Abstract
Neutrophils, migrating to the site of infection, are able to release serine proteases after being activated. These serine proteases comprise cathepsin G (CatG), neutrophil elastase protease 3 (PR3), and neutrophil serine protease 4 (NSP4). A disadvantage of the uncontrolled proteolytic activity of proteases is the outcome of various human diseases, including cardiovascular diseases, thrombosis, and autoimmune diseases. Activity-based probes (ABPs) are used to determine the proteolytic activity of proteases, containing a set of three essential elements: Warhead, recognition sequence, and the reporter tag for detection of the covalent enzyme activity–based probe complex. ...
Description
Keywords
immune cells, serine proteases, activity-based probes
Citation
Burster T, Gärtner F, Knippschild U and Zhanapiya A (2021) Activity-Based Probes to Utilize the Proteolytic Activity of Cathepsin G in Biological Samples. Front. Chem. 9:628295. doi: 10.3389/fchem.2021.628295