CRYOGELS CONTAINING POLYELECTROLYTE COMPLEX FOR TISSUE ENGINEERING
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Date
2020
Authors
Berillo, D.
Zheng, Y.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
The design of scaffolds for tissue engineering is an important task. The focus of our research is the
design of various biocompatible scaffolds based on natural polymers such as gelatin, chitosan and casein
using cryogelation technique.1-4 Cryogelation involves a process of the formation of macroporous
polymer systems, so called cryogels, with well-developed 3D structure of interconnected pores. Typically,
cryogels have porosity of 90% and macrochannels of 30-200 μm in size, depending on the preparation
conditions. Cryogels has ability of unrestricted penetration of solutes as well as high surface area for
attachment and proliferation of mammalian cells.2,3 Previously, the preparation of gelatin based cryogel
was performed in environmentally friendly way using enzymatic reaction under cryoconditions.3 We
used dextran dialdehyde as a mild nontoxic cross-linker and additional physical cross-linking via formation
of polyelectrolyte complex(PEC) between oppositely charged groups of polymers. The advantage of
PEC based scaffold preparation is the simultaneous existence of positive and negative charges on the
surface at physiological pH, facilitating attachment of tissue components via electrostatic interactions,
that is favourable for a tissue engineering2. Human hepatic epithelial cell line and fibroblasts were used
for evaluation of biocompatibility. It is important to mention that the gelatin type A and B significantly
different and therefore affecting the migration, proliferation of cells and also microscopic morphology
of the material. This phenomenon may be related to different chemical composition effecting isoelectric
point of gelatin. In the present study gelatin was utilised from cold skin fish and bovine type A were used.
The PEC scaffold containing gelatin from fish exhibited better fibroblast growth compare to cryogels
based on only gelatin and aldehyde dextran. The same composition cryogels based on gelatin(bovine A)
and dextran dialdehyde revealed proliferation of hepatocytes inside of the material, whereas hepatocytes
formed clusters on the surface of the PEC cryogel.
References:
1. Progress in the development of chitosan-based biomaterials for tissue engineering and biomedical
application. Biomolecules (2019).
2.Oxidized dextran as crosslinker for chitosan cryogel scaffolds and formation of polyelectrolyte complexes
between chitosan and gelatin. Macromolecular bioscience(2012).
3.Biocompatible scaffolds for regenerative medicine. International (Int) journal of biological macromolecules(
2018).
Description
Keywords
bio-materials, proliferation and migration of cells, scaffolds, bio-compatibility, Research Subject Categories::TECHNOLOGY