PERIOSTIN IN INFLAMMATORY BOWEL DISEASE (IBD) DEVELOPMENT AND SYNERGISTIC EFFECTS MEDIATED VIA CCL5

Abstract

Inflammatory bowel disease (IBD) is characterized by chronic inflammation in any part of the gastrointestinal tract (Ulcerative colitis and Crohn’s disease) and leads to severe damage mostly of small and/or large intestines. Periostin is both, an extracellular and a matricellular protein that involved in formation and functioning of several different organs and biologically important= signaling pathways. The precise role of periostin in IBD is still unclear, although the impact of protein in the intestinal inflammation has been demonstrated. CCR5, a seven transmembrane G-coupled receptor, is potentially associated with chronic inflammation, leading to significant consequences. The mode of action from CCR5 in IBD may be linked to effects mediated by periostin or act independently. We investigated the patterns of Periostin and CCR5 expression in tissue samples from wild type and Periostin knockout DSS and TNBS treated mice employing immunohistochemical staining. The absence of periostin seems to reduce the amount of the CCR5 expression in animal models of colitis (DSS and TNBS). The acute colitis was induced by oral DSS and rectal TNBS administration. An experiment involving live mice and the use of CCL5 5p12 5m and Maraviroc injections and oral consumption of Maraviroc was carried out and some results obtained, but further analysis is still ongoing.