Adipose-derived perivascular stem cells promote sensorimotor recovery after ischemic stroke in rats

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dc.contributor.authorOgay, Vyacheslav
dc.contributor.authorKumasheva, Venera
dc.contributor.authorBaidosova, Sholpan
dc.contributor.authorLi, Yelena
dc.contributor.authorShpekov, Azat
dc.contributor.authorMakhambetov, Yerbol
dc.contributor.authorKaliyev, Assylbek
dc.contributor.authorZhetpisbayev, Berik
dc.contributor.authorOlzhayev, Farkhad
dc.contributor.authorRamankulov, Yerlan
dc.creatorVyacheslav, Ogay
dc.date.accessioned2017-12-20T08:14:34Z
dc.date.available2017-12-20T08:14:34Z
dc.date.issued2017-08-30
dc.description.abstractIn this study, we test a novel hypothesis that treatment of stroke with adipose-derived perivascular stem cells (PSCs) promote sensorimotor recovery after stroke in rats. Rat PSCs were isolated from subcutaneous adipose tissue by a FACS Aria cell sorter using antibodies against CD146, CD31, CD34 and CD45. Isolated and propagated PSCs exhibited fibroblast-like morphology and significantly expressed both mesenchymal stem cells and pericyte markers such as CD73, CD90, CD105, CD146, NG2 and PDGFR-β. Adult male Wistar rats were subjected to 2 h of middle cerebral artery occlusion (MCAo) followed by femoral vein injection of PSCs (3 × 106 cells) 24 h later. The rats were sacrificed at 28 days after stroke and immunohistochemistry were performed to identify angiogenesis. It was observed that intravenous injection of PSCs significantly promoted sensomotoric recovery in stroke rats compared with PBS-treated controls. Number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in MCAo rats treated with PSCs.en_US
dc.identifierDOI:10.1016/j.jbiotec.2017.06.1196
dc.identifier.citationVyacheslav Ogay, Venera Kumasheva, Sholpan Baidosova, Yelena Li, Azat Shpekov, Yerbol Makhambetov, Assylbek Kaliyev, Berik Zhetpisbayev, Farkhad Olzhayev, Yerlan Ramankulov, Adipose-derived perivascular stem cells promote sensorimotor recovery after ischemic stroke in rats, In Journal of Biotechnology, Volume 256, Supplement, 2017, Page S116en_US
dc.identifier.issn01681656
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S016816561731492X
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/2959
dc.language.isoenen_US
dc.publisherJournal of Biotechnologyen_US
dc.relation.ispartofJournal of Biotechnology
dc.rights.licenseCopyright © 2017 Published by Elsevier B.V.
dc.titleAdipose-derived perivascular stem cells promote sensorimotor recovery after ischemic stroke in ratsen_US
dc.typeArticleen_US
elsevier.aggregationtypeJournal
elsevier.coverdate2017-08-30
elsevier.coverdisplaydate30 August 2017
elsevier.identifier.doi10.1016/j.jbiotec.2017.06.1196
elsevier.identifier.eid1-s2.0-S016816561731492X
elsevier.identifier.piiS0168-1656(17)31492-X
elsevier.issue.nameEuropean Biotechnology Congress 2017 held in Dubrovnik, Croatia during 25 - 27 May 2017
elsevier.openaccess0
elsevier.openaccessarticlefalse
elsevier.openarchivearticlefalse
elsevier.startingpageS116
elsevier.volume256
workflow.import.sourcescience

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