DEVELOPING A SYSTEM FOR ANALYZING THE DOWNSTREAM EFFECTS OF CCL5 DERIVATIVES ON THE CCR5 PATHWAY

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dc.contributor.authorMustafa, Kamshat
dc.date.accessioned2024-06-25T09:39:01Z
dc.date.available2024-06-25T09:39:01Z
dc.date.issued2023
dc.description.abstractBackground: Various types of diseases are known to be influenced by the ССL5/ССR5 pathway, including cancers and HIV. Inhibiting the attachment of CCL5 to CCR5 has shown promise in preclinical studies as a way to suppress the growth of the tumor and improve outcomes in cancer patients. In this study, we want to observe how p21, p53, and Mdm2 protein expressions that play important roles in cell growth will change with different modifications of CCL5. We aim to check the level of expression of these proteins in the cell line derived from brеаst саnсеr - МСF-7. Маtеrіаls & Меtһоds: МСF-7, МDА-МВ-231, СНО/CCR5, and СНО/WT cell lines were cultured for further RNA isolation, RT-PCR, and qPCR experiments. RNA isolation and RT-PCR were performed in order to check whether the expected genes exist. In order to check the expression levels of three target proteins quantitative PCR was done. Results: RT-PCR interpretation on a gel showed that cell lines express p21, Mdm2, and GAPDH genes, but failed to show p53. Only MDA-MB-231 cells were positive for CCR5 on a gel. qPCR analysis failed to determine Ct values for GAPDH and p53. That is why we were unable to calculate gene expression values for target genes and the experiment should be repeated.en_US
dc.identifier.citationMustafa, K. (2023). Developing a system for analyzing the downstream effects of CCL5 derivatives on the CCR5 pathway. Nazarbayev University School of Medicineen_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/8004
dc.language.isoenen_US
dc.publisherNazarbayev University School of Medicineen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectbreast canceren_US
dc.subjectCCL5 derivativesen_US
dc.subjectCCR5en_US
dc.subjectp21, p53, Mdm2en_US
dc.subjectprotein expressionen_US
dc.subjectType of access: Restricteden_US
dc.titleDEVELOPING A SYSTEM FOR ANALYZING THE DOWNSTREAM EFFECTS OF CCL5 DERIVATIVES ON THE CCR5 PATHWAYen_US
dc.typeCapstone Projecten_US
workflow.import.sourcescience

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