APOPTOTIC EFFECT OF ELECTROMAGNETIC FIELD ON ASTROCYTES
Loading...
Date
2020
Authors
Begimbetova, Dinara
Baiskhanova, Dinara
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
Introduction: Over the past decade, the use of mobile phones in many countries of the world has
increased by almost 100%, and this increase has raised concerns about possible risks to human health.
In addition to the emission of mobile phones and, effects of base stations of mobile communication are
also possible. Compared to other organs, the brain is subject to relatively high specific absorption rates
due to the close proximity of the cell phone device to the user’s head. The data show that even ultra-low
EMF is powerful enough to disrupt biological processes at the cellular level, but more research is needed
in this direction to understand the basic mechanisms.
Methods: The experiments used primary cortical astrocytes obtained using the standard procedure
for stratification of cells from abortion material of laboratory rats Rattus Norvegicus. Portable Faraday
cage with electromagnetic signals up to 6 GHz. EMFaraCage® (Standard EMFaraCage® RF Shielding Test
Enclosure) was used with the EMF emitted by the antenna. Cells were exposed to EMFs with a power of
900 and 1800 MHz at time intervals of 10, 30, 60, minutes, and 24 and 48 hours. Early apoptosis levels using
CellEvent ™ Caspase-3/7 Green Detection Reagent (Invitrogen) fluorescence reagents and Propidium
Iodid Blue (Invitrogen) late apoptosis on a Cytation 5 multifunction cell imaging reader.
Results: The experimental results showed a statistically insignificant increase in the frequency of early
and late apoptosis when exposed to EMF with a power of 900 MHz at all time intervals. When exposed to
an EMF with a frequency of 1800 MHz, the activation of Annexin Caspase 3/7 and propidium iodide was
statistically insignificant in temporary exposures of 10, 30, 60, minutes, and 24 hours. An increase in the
level of early apoptosis when exposed to EMF with a frequency of 1800 MHz for 48 hours was statistically
significant (p ≤ 0.01). The level of late apoptosis (propidium iodide) of this exposure remained at the level
of intact exposure. Thus, a significant negative effect of apoptosis was noted at its early stage with maximum
exposure to EMF. To further study the mechanism of induction of apoptosis of EMF, the genotoxic
effect of EMF will be investigated.
Acknowledgements: This work was supported by grant to Dinara Begimbetova from MES RK grant
AP05133910, “Bio-effects of Electromagnetic Radiation from Mobile Phones and Wi-Fi on DNA of brain
cells”.
Description
Keywords
apoptosis, electromagnetic field, EMF, Research Subject Categories::MEDICINE