Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16
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Date
2015-08-01
Authors
Shevtsov, Alexandr
Ramanculov, Erlan
Shevtsova, Elena
Kairzhanova, Alma
Tarlykov, Pavel
Filipenko, Maxim
Dymova, Maya
Abisheva, Gulzada
Jailbekova, Aygul
Journal Title
Journal ISSN
Volume Title
Publisher
Infection, Genetics and Evolution
Abstract
Abstract Brucellosis is an endemic disease in Central Asia characterized by high infection rates in humans and animals. Currently, little is known about the genetic diversity of Brucella spp. circulating in the region, despite the high prevalence of brucellosis. This study aimed to analyze the genetic diversity of Brucella melitensis and Brucella abortus strains circulating in the Republic of Kazakhstan. We genotyped 128 B. melitensis and 124 B. abortus strains collected in regions with the highest prevalence of brucellosis. Genotyping was performed using multi-locus variable-number tandem-repeat analysis (MLVA). Analysis of a subset of 8 loci (MLVA-8) of 128 B. melitensis strains identified genotypes 42 (n=108), 43 (n=2), and 63 (n=19) related to the ‘East Mediterranean’ group. An MLVA-16 assay sorted 128 B. melitensis strains into 25 different genotypes. Excluding one variable locus, MLVA-15 of B. melitensis was distinct from strains originating in the Mediterranean region; however, 77% of them were identical to strains isolated in China. A minimum spanning tree for B. melitensis using MLVA-15 analysis clustered the local strains together with strains previously collected in China. MLVA-8 analysis of 124 B. abortus strains identified them as genotype 36, suggesting Eurasian distribution of this lineage. Complete MLVA-16 assay analysis clustered the strains into five genotypes, revealing little diversity of B. abortus when compared on the global scale. A minimum spanning tree for B. abortus obtained using MLVA-15 analysis clustered the 2 most prevalent genotypes (n=117) together with strains previously collected in China. Thus, MLVA analysis was used to characterize 252 strains of Brucella collected in Kazakhstan. The analysis revealed genetic homogeneity among the strains. Interestingly, identical MLVA-15 profiles were found in seemingly unrelated outbreaks in China, Turkey, and Kazakhstan. Further analysis is needed for better understanding of the epidemiology of brucellosis in Asia.
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Keywords
Multi-locus variable number tandem repeat analysis (MLVA), Brucella melitensis, Brucella abortus, Genotyping, Kazakhstan
Citation
Alexandr Shevtsov, Erlan Ramanculov, Elena Shevtsova, Alma Kairzhanova, Pavel Tarlykov, Maxim Filipenko, Maya Dymova, Gulzada Abisheva, Aygul Jailbekova, Dinara Kamalova, Andrei Chsherbakov, Samat Tulegenov, Assel Akhmetova, Igor Sytnik, Talgat Karibaev, Kasim Mukanov, Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16, In Infection, Genetics and Evolution, Volume 34, 2015, Pages 173-180